简介

GenJet™体外DNA转染试剂是一种新结构类型的DNA转染试剂。该转染试剂为阳离子聚合物和脂质体的杂合体，具有转染效率高、重复性好、毒性低和稳定性极强的特点(室温条件下可保存18个月!)，目前该DNA转染试剂为我公司独家产品。GenJet™转染试剂用于哺乳动物细胞的转染，如肿瘤细胞系(常见的细胞有HEK293, CHO, COS-1, COS-7, Hela, NIH3T3等)和原代培养的细胞以及昆虫细胞sf9, sf21等。GenJet™试剂，1.0 ml，可用于24孔板转染333次或者6孔板166次转染操作。

应用
- 将DNA导入哺乳动物细胞和昆虫细胞
- 同病毒协同转染难转哺乳动物细胞，可显著增强病毒的转染效率(可增高200倍)

特点
- 对哺乳动物细胞有极高的转染效率
- 可获得极高滴度的病毒(如慢病毒， AAV等)
- 对大片段DNA分子(大到180 kb)有同样高的转染效率
- 对单个DNA或多个DNA共转染具有同样好的效率
- 可获得高水平的重组蛋白表达
- 不受血清和抗生素的影响
- 对难转原代细胞有较好的转染效率

保存条件
该产品于4~23°C保存，可稳定18个月。

GenJet™转染试剂哺乳动物细胞转染谱

 

Cell Lines	Transfection Efficiency (% GFP)	Cell Lines	Transfection Efficiency (% GFP)
3LL B16-F10 BAEC BHK-21 Ca Ski CaCo-2 CHO HCS-2/8 HEK-293 HeLa HLMEC H-MVEC Huh-7D12 ATT20 SK-N-SH McArdle 7777 Hep3D SHEP MDA-MB-231 3T3-442A COS-7 CV-1 D 407 DHD Pro.b LS180	51% 72% 52% 86% 78% 32% 83% 42% 80-90% 81% 50-65% 49% 22% 38% 20% 60% 78% 71% 38% 10% 60-70% 53% 62% 51% 46%	K562 L929 MCF-7 MDCK Neuro2A NIH 3T3 PC12 SH-SY5Y SiHa SKOV3 HUVEC IGROV1 Jurkat 6CSFMEo WEHI 231 SAOS-2 SN56 MC3T3-E1 BT474 C2C12 Primary melanocyte Hela-S3 A549 Hep G2	22% 52% 51% 52% 72% 88% 34% 14% 62% 52% 51% 21% 5%-15% 73% 25% 52% 69% 70% 46% 46% 46% 78% 78% 58%

GenJet体外DNA转染试剂同市场上知名品牌的比较

Exceptional high titers of lentivirus generated from 293F cells. Three DNAs co-transfected with GenJet™ In Vitro DNA Transfection Reagent (left panel) in comparison of LipoFectamine 2000™ (L2K, right panel). 100 microliter virus supernatant from 293F cells were used to counter-infected 293F cells which was then passed FACS. The numbers at the upper right corner indicate the percentage of transduced cells. The titers of lentivirus generated with GenJet™ and L2K were quantified to be 7.9x10⁶ and 3.1x10⁶ tu/ml respectively

Primary Murine Skin Fibroblast transfected with pEGFP-C1 plasmid using GenJet™ In Vitro DNA Transfection Reagent (upper panel) and the most popular brand product FuGene™ HD (lower panel). The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging (left panel) and FITC imaging (right panel) 24 hours post-transfection

Primary Murine Keratinocytes transfected with pEGFP-C1 plasmid using GenJet™ In Vitro DNA Transfection Reagent (upper panel) and one of the most popular brand product FuGene™ HD (lower panel). The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging (left panel) and FITC imaging (right panel) 24 hours post-transfection

Comparison of cytotoxicity of GenJet™ DNA In Vitro Transfection Reagent with L2K™ on primary murine skin fibroblast. The primary murine fibroblast was incubated with the indicated transfection reagents/pEGFP-C1 (DNA) complexes above for 4 hours in serum-free DMEM High Glucose medium followed by replacement of complete serum-containing medium. The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging 24 hours post transfection

Technical Information & Datasheet
- GenJet™ In Vitro DNA Transfection Reagent FAQ
- GenJet™ In Vitro DNA Transfection Insert Sheet
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