预优化DNA转染试剂-Neuro-2A
英文名称： GenJet™ In Vitro DNA Tranfection Reagent for Neuro-2A Cells
型号:null    产品货号： SL100489-N2A
价格:请致电：010-57128832,18610462672
品牌: 美国

Description

GenJet™ DNA In Vitro Tranfection Reagent for Neuro2A is pre-optimized for transfecting Neuro2A cells. Refer to the following optimal transfection conditions for maximal transfection efficiency on Neuro2A cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 100 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium 5 Hours After Transfection
Maximal Efficiency 

Transfection Results:
Reporter Gene
Plasmid
Efficiency (GFP %)


~90%
DMEM with 4.5 g/L glucose, 10% FBS
3:1
Serum-free DMEM with 4.5 g/L glucose
15 minutes at RT
Yes
Yes
48 hours


EGFP
pEGFP-N3 (CMV promoter) 
89%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer


A Picture Showing Transfection Efficiency of GenJet™ Reagent on Neuro2A Cells.  Neuro2A cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™ and visualized by a Nikon Eclipse Fluorescence microscope with DIC phase (left panel) and FITC (right panel) imaging 36 hours post transfection 

Data Sheet.pdf