Description:
GenMute™ siRNA Transfection Reagent for Primary Keratinocytes is pre-optimized for transfecting siRNA to primary keratinocytes. Primary keratinocytes are known for notorious resistance for DNA and siRNA transfections. With the pre-optimized transfection conditions, average 90% silencing can be obtained.

Size & content:
- GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 20 pmoles siRNA or miRNA mimics in 24-well plate
- GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 ml to make 40 ml working solution

Refer to the following optimal transfection conditions for maximal silencing on primary keratinocytes. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.

Summary of Optimal Transfection Conditions: Cell culture medium Confluency of cell on the day of transfection Optimal siRNA concentration Diluent for siRNA and GenMute™ Reagent Incubation Time to Form GenMute™/siRNA Complex Maximal Silencing Efficiency  Transfection Results: Reporter Gene Silencing Efficiency (% )

DMEM with 4.5 g/L glucose, 10% FBS ~70% 40 nM 1xGenMute™ Transfection Buffer ~15 minutes at RT 48 hours Renilla Luciferase 90%

Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

Data Sheet & Protocol.pdf