英文名称： GQH-IIR
人先天性免疫应答定量PCR芯片试剂盒
ScienCells GeneQuery™ Human Innate Immune Response qPCR Array Kit (GQH-IIR) facilitates gene expression profiling of key genes in the human innate immune system. The innate immune response is activated upon microbial infection or foreign substance presentation and includes activation of the complement system and adaptive immune systems. A separate array is available for analyzing the adaptive immune response. Brief examples of how included genes may be grouped according to function are shown below:
- Bacteria response: TLR1, TLR5, NOD1, LY96, TRAM1
- Virus response: TLR3, DDX58, IFIH1, MYD88, IRAK4
- Self recognition: TLR2, TLR4, TLR7, TLR8, CLEC4E
- Inflammasome component: CASP1, CASP5, PYCARD, NLRP1
- Complement system component: C3, MBL2, MASP1, CFB, CD46
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Innate Immune Response qPCR Array Plate Layout* (8 controls in Bold and Italic)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | ATF3 | CASP5 | CLEC6A | IFIH1 | IRAK1 | LYZ | NAP1L1 | OTUD5 | TICAM1 | TLR5 | TRAF6 | β-actin |
| B | C3 | CD46 | CLEC7A | IFNA1 | IRAK2 | MAP3K7 | NFAM1 | PYCARD | TICAM2 | TLR6 | TRAM1 | GAPDH |
| C | C5 | CFB | DDX58 | IFNB1 | IRAK3 | MAPK8 | NFATC1 | RIPK2 | TIRAP | TLR7 | TRAM2 | LDHA |
| D | C6 | CFD | DHX58 | IFNG | IRAK4 | MASP1 | NFKB1 | RNF125 | TLR1 | TLR8 | TRIM25 | NONO |
| E | C7 | CFH | EYA4 | IL18 | IRF3 | MASP2 | NFKBIE | SYK | TLR10 | TLR9 | TYROBP | PPIH |
| F | C8A | CFP | FADD | IL1A | IRF7 | MAVS | NLRP1 | TAB2 | TLR2 | TNF | UBE2N | GDC |
| G | CARD9 | CHUK | HCST | IL1B | JUN | MBL2 | NOD1 | TAB3 | TLR3 | TRADD | UBE2V1 | PPC |
| H | CASP1 | CLEC4E | HMGB1 | IL6 | LY96 | MYD88 | NOD2 | TBK1 | TLR4 | TRAF3 | UNC93B1 | NTC |
*gene selection may be updated based on new research and development
| Catalog No. | GK039 |
|---|---|
| Country of Manufacture | United States |
| Product Code | GQH-IIR |
| Size/Quantity | One 96-well plate, one 20ul reaction per well |
| Product Use | GQH-IIR is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures. |
| Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| Shipping Info | The product is shipped at ambient temperature. Upon receipt, the plate should be stored at 4°C and is good for up to 12 months. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| References | [1] Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F. (2002) "Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes." Genome Biol. 3(7): 1-12. |
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