英文名称： GQH-BRS
试剂级别： 试剂级
人B细胞受体信号通路定量PCR芯片试剂盒描述：
ScienCells GeneQuery™ Human B Cell Receptor Signaling Pathway qPCR Array Kit (GQH-BRS) is designed to facilitate gene expression profiling of 88 key genes involved in B cell receptor signaling pathway. The B cell receptor signaling pathway is important for antibody production, B cell proliferation and differentiation. Brief examples of how included genes may be grouped according to their functions are shown below:
- B cell receptor complex components: CD79A, CD79B
- Co-inhibitory receptors: CD22, CD72, FCGR2B
- Co-stimulators: CD81, CR2, IFITM1
- Expression adaptor proteins: BLNK, GAB2, GRB2, CARD11
- Kinases: LYN, SYK, PI3Ks
- Phosphatases: INPP5D, PTPN6
- MAPK pathway: KRAS, HRAS, NRAS, RAF1, MAP2K1, MAPK1, MAPK3
- PI3K/AKT pathway: PI3Ks, AKT1, AKT2
- IKK/NFKB pathway: NFKBs, PRKCB, PLCG2, DAG1, BCL10, CARD11, MAP3K7, MALT1, CHUK, IKBKB, IKBKG
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human B Cell Receptor Signaling Pathway qPCR Array Plate Layout*
(8 controls in Bold and Italic)
|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| A | AKT1 | CD22 | DAPP1 | GSK3B | INPP5D | MAP2K1 | MYC | NFKBIE | PIK3R3 | PRKCD | RELA | β-actin |
| B | AKT2 | CD72 | ELK1 | GTF2I | INPPL1 | MAP3K7 | NCK1 | NRAS | PIP5K1A | PTPN18 | SH3BP2 | GAPDH |
| C | ATF2 | CD79A | FCGR2B | HCLS1 | IRF4 | MAP4K1 | NFATC1 | PDPK1 | PIP5K1C | PTPN6 | SHC1 | LDHA |
| D | BCL10 | CD79B | FOS | HRAS | JUN | MAPK1 | NFATC2 | PIK3AP1 | PLCG2 | RAC1 | SOS1 | NONO |
| E | BLNK | CD81 | FYN | IFITM1 | KRAS | MAPK3 | NFATC3 | PIK3CA | PPP3CA | RAC2 | SOS2 | PPIH |
| F | BTK | CHUK | GAB2 | IKBKB | LAT2 | MAPK4 | NFKB1 | PIK3CB | PPP3CB | RAF1 | SYK | GDC |
| G | CARD11 | CR2 | GRB2 | IKBKG | LYN | MAPK9 | NFKBIA | PIK3R1 | PPP3R1 | RASGRP3 | TEC | PPC |
| H | CBL | DAG1 | GSK3A | ILF2 | MALT1 | MEF2D | NFKBIB | PIK3R2 | PRKCB | REL | VAV2 | NTC |
* gene selection may be updated based on new research and development
| Catalog No. | GK020 |
|---|---|
| Country of Manufacture | United States |
| Product Code | GQH-BRS |
| Size/Quantity | One 96-well plate, one 20ul reaction per well |
| Product Use | GQH-BRS is for research use only. It is not approved for human or animal use or for application in clinical or in vitro diagnostic procedures. |
| Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| Shipping Info | Ambient temperature. |
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