英文名称： GQH-BCC
Sciencell公司产品由上海中乔新舟生物科技有限公司优质供应
美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力
于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养
基的研究和开发，在全球拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高
的文献引用率。凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。
ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21
种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。
确保了实验结果的重复性和连贯性。
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人基底细胞癌定量PCR芯片试剂盒描述：
ScienCells GeneQuery™ Human Basal Cell Carcinoma qPCR Array Kit (GQH-BCC) is designed to facilitate gene expression profiling of key genes involved in (i) basal cell carcinoma (BCC) biology and carcinogenesis; (ii) targeted BCC therapies; and (iii) hedgehog signaling pathway. 88 genes are selected in this kit based on database and literature research.
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ qPCR Array Kit Controls
Each GeneQuery™ plate contains eight controls (Figure 1).
- Five target housekeeping genes (β-actin, GAPDH, LDHA, NONO, and PPIH), which enable normalization of data.
- The Genomic DNA (gDNA) Control (GDC) detects possible gDNA contamination in the cDNA samples. It contains a primer set targeting a non-transcribed region of the genome.
- Positive PCR Control (PPC) tests whether samples contain inhibitors or other factors that may negatively affect gene expression results. The PPC consists of a predispensed synthetic DNA template and a primer set that can amplify it. The sequence of the DNA template is not present in the human genome, and thus tests the efficiency of the polymerase chain reaction itself.
- The No Template Control (NTC) is strongly recommended, and can be used to monitor the DNA contamination introduced during the workflow such as reagents, tips, and the lab bench.
GeneQuery™ Human Basal Cell Carcinoma qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| A | APC | CD151 | CSNK1G2 | EIF4E | FZD5 | GSK3B | LRP2 | RAB23 | TCF7L1 | WNT11 | WNT5B | β-actin |
| B | APC2 | CDH1 | CSNK1G3 | FAT2 | FZD6 | HHATL | MC1R | ROR2 | TCF7L2 | WNT16 | WNT7A | GAPDH |
| C | AXIN1 | CEBPA | CTNNB1 | FBXW11 | FZD7 | HHIP | NET1 | SHFM1 | TGFBR2 | WNT2 | WNT7B | LDHA |
| D | AXIN2 | CSNK1A1 | DHH | FZD1 | FZD8 | IFNA1 | PARP1 | SHH | TP53 | WNT2B | WNT8A | NONO |
| E | BMP2 | CSNK1A1L | DVL1 | FZD10 | FZD9 | IHH | PRKACA | SMO | TXN | WNT3 | WNT8B | PPIH |
| F | BMP4 | CSNK1D | DVL2 | FZD2 | GAS1 | KLF4 | PRKACB | STAT2 | WNT1 | WNT3A | WNT9A | GDC |
| G | BTRC | CSNK1E | DVL3 | FZD3 | GLI2 | LEF1 | PRKACG | SUFU | WNT10A | WNT4 | WNT9B | PPC |
| H | CASP3 | CSNK1G1 | EGF | FZD4 | GLI3 | LOC400927 | PTCH1 | TCF7 | WNT10B | WNT5A | ZIC2 | NTC |
* gene selection may be updated based on new research and development
| Catalog No. | GK015 |
|---|---|
| Country of Manufacture | United States |
| Product Code | GQH-BCC |
| Size/Quantity | One 96-well plate, one 20ul reaction per well |
| Product Use | GQH-BCC is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures. |
| Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| Shipping Info | The product is shipped at ambient temperature. Upon receipt, the plate should be stored at 4°C and is good for up to 12 months. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| References | [1] Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F. (2002) "Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes." Genome Biol. 3(7): 1-12. |
相关产品目录：
热烈祝贺上海中乔新舟生物科技有限公司成为美国Sciencell公司在中国正规一级代理商：
Sciencell公司产品由上海中乔新舟生物科技有限公司优质供应
近几年的新药研发成功率在逐年下降，其根本原因之一就是传统的药物筛选系统是建立在只具有30-40%
人类基因群的一系列细胞株上。这样一个有“缺陷型”药物筛选系统所产生的药物用于人体上就会出现许
多致命的弱点和不完整性。新一代药物筛选系统是含有一系列近乎完整的人类基因群的原代细胞株。而利
用这些原代细胞株所甄别和筛选出来的候选药物，其诊治人类疾病的成功几率将大大增加。这样不但大大
节省了新药的开发成本，而且将极大地提高人类的健康质量。这些产品从根本上提高了全球生命医学研究、
人类重要疾病药物研发、新药研发的成功率。
所以上海中乔新舟生物科技有限公司致力于提供优质原代细胞产品和完善的售后服务。
Sciencell公司部分原代细胞目录（如需要其他细胞资料请发邮件至 wwwfudan@163.com索取）：
上海中乔新舟专长于为生物医药领域的医疗机构、研究中心、企业、临床医生等提供课题设计、基金联合申请、实验
技术服务、论文相关服务、采购外包等整体服务，目前已经成长为国内领先的转化医学外包品牌。
中乔新舟的PI团队已经发展到专职PI 20人，联席PI 312人，其中大多数拥有海外背景。截止到2015年以PI或联席PI为
第一作者发表SCI 论文1682 篇（总IF 值为5721.82，其中影响因子IF>5.0 的论文216 篇，IF>10.0论文32 篇）。
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