英文名称： GQH-TRS
试剂级别： 试剂级
人T细胞T细胞受体信号通路定量PCR芯片试剂盒描述：
ScienCells GeneQuery™ Human T Cell Receptor Signaling Pathway qPCR Array Kit (GQH-TRS) is designed to facilitate gene expression profiling of 88 key genes involved in T cell receptor signaling pathway. Brief examples of how included genes may be grouped according to their functions are shown below:
- T cell receptor complex components: CD3D, CD3E, CD3G
- Co-receptors: CD4, CD8A, CD8B
- Co-inhibitors: PDCD1, CTLA4
- Co-stimulators: ICOS, CD28
- Kinases: FYN, ZAP70
- MAPK pathway: KRAS, HRAS, NRAS, RAF1, MAP2K1, MAPK1, MAPK3
- PI3K/AKT pathway: PI3Ks, AKT1, AKT2
- IKK/NFKB pathway: NFKBs, DAG1, BCL10, CARD11, MAP3K7, MALT1, CHUK, IKBKB, IKBKG
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human T Cell Receptor Signaling Pathway qPCR Array Plate Layout*
(8 controls in Bold and Italic)
|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| A | AKT1 | CD274 | CD8A | DYRK1A | HRAS | MALT1 | NCK1 | NFKBIE | PDPK1 | PPP3CB | RELA | β-actin |
| B | AKT2 | CD28 | CD8B | DYRK2 | ICOS | MAP2K1 | NCK2 | NRAS | PIK3CA | PPP3R1 | SOS1 | GAPDH |
| C | BCL10 | CD3D | CDC42 | FOS | IKBKB | MAP2K7 | NFATC1 | NRON | PIK3CB | PRKCA | SOS2 | LDHA |
| D | CALM1 | CD3E | CDK4 | FYN | IKBKG | MAP3K7 | NFATC2 | PAK1 | PIK3R1 | PRKCQ | TEC | NONO |
| E | CARD11 | CD3G | CHUK | GRAP2 | ITK | MAP3K8 | NFATC3 | PAK2 | PIK3R2 | PTCRA | TNF | PPIH |
| F | CBL | CD4 | CTLA4 | GRB2 | JUN | MAPK1 | NFKB1 | PAK3 | PIK3R3 | PTPN6 | VAV1 | GDC |
| G | CBLB | CD80 | DAG1 | GSK3A | KRAS | MAPK3 | NFKBIA | PAK4 | PLCG1 | RAF1 | VAV2 | PPC |
| H | CBLC | CD86 | DLG1 | GSK3B | LCP2 | MAPK9 | NFKBIB | PDCD1 | PPP3CA | RASGRP1 | ZAP70 | NTC |
* gene selection may be updated based on new research and development
| Catalog No. | GK021 |
|---|---|
| Country of Manufacture | United States |
| Product Code | GQH-TRS |
| Size/Quantity | One 96-well plate, one 20ul reaction per well |
| Product Use | GQH-TRS is for research use only. It is not approved for human or animal use or for application in clinical or in vitro diagnostic procedures. |
| Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| Shipping Info | Ambient temperature. |
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