ScienCells GeneQuery™ human apoptosis qPCR array kit (GQH-APO) is designed to facilitate gene expression profiling of key genes involved in human apoptosis. 88 genes are selected in this kit based on public database and literature research.      

GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Apoptosis qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ABL1

BAG1

BIRC1

BRAF

CASP6

DFFA

IL1RAP

MYD88

PIK3R3

RELA

TNFRSF9

β-actin

B

AIFM1

BAG3

BIRC2

CAPN1

CASP9

DIABLO

IL3

NGF

PPP3CA

RIPK1

TNFSF10

GAPDH

C

AKT1

BCL10

BIRC3

CAPN2

CD40

ENDOG

IRAK2

NOD1

PPP3CB

RIPK2

TP53

LDHA

D

AKT2

BCL2

BIRC5

CASP1

CFLAR

FAS

IRAK3

NOL3

PPP3R1

TNF

TP53BP2

NONO

E

AKT3

BCL2L1

BIRC6

CASP10

CHUK

HRK

IRAK4

PIK3CB

PRKAR1A

TNFRSF10B

TRADD

PPIH

F

APAF1

BCL2L10

BNIP2

CASP2

CIDEA

IGF1R

LTBR

PIK3CG

PRKAR2B

TNFRSF10D

TRAF2

GDC

G

ATM

BID

BNIP3

CASP3

CRADD

IL1B

MAP3K14

PIK3R1

PRKX

TNFRSF11B

TRAF3

PPC

H

BAD

BIK

BNIP3L

CASP4

DAPK1

IL1R1

MCL1

PIK3R2

PYCARD

TNFRSF1B

XIAP

NTC

 

* gene selection may be updated based on new research and development