英文名称： GQH-TCD
试剂级别： 试剂级
人甲状腺癌和疾病定量PCR芯片试剂盒描述：
ScienCells GeneQuery™ Human Thyroid Cancer and Disorders qPCR Array Kit (GQH-TCD) is designed to facilitate gene expression profiling of key genes involved in (i) the development of various types of thyroid cancer (papillary, follicular, and Hürthle cells); (ii) cell cycle regulation in the progression of thyroid cancer; and (iii) thyroid disorders, such as autoimmune thyroiditis and Hashimotos thyroiditis. 88 genes are selected in this kit based on database and literature research.
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Thyroid Cancer and Disorders qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| A | AIRE | CCNE2 | CDK4 | FAS | HLA-DPA1 | IFNA2 | KRAS | MYC | PIK3CA | RNF139 | TFG | β-actin |
| B | AKT1 | CD28 | CDKN1A | FOXE1 | HLA-DQA1 | IFNA4 | LEF1 | NCOA4 | PPARG | RXRA | TP53 | GAPDH |
| C | BRAF | CD40 | CTLA4 | GOLGA5 | HLA-DRA | IFNA5 | LGALS3 | NDUFA13 | PRF1 | RXRB | TPM3 | LDHA |
| D | C8orf4 | CD80 | CTNNB1 | HLA-A | HLA-E | IFNA6 | MAP2K1 | NKX2-1 | PRKAR1A | RXRG | TPR | NONO |
| E | CALCA | CD86 | DIO2 | HLA-B | HLA-F | IFNG | MAP2K2 | NNMT | PTEN | SERPINA7 | TRIM24 | PPIH |
| F | CCDC6 | CDC23 | EGF | HLA-C | HRAS | IL2RA | MAPK1 | NRAS | PTTG1 | TCF7 | TRIM27 | GDC |
| G | CCNB1 | CDH1 | ERC1 | HLA-DMA | ICAM1 | KDR | MAPK3 | PAX8 | RASSF1 | TCF7L1 | TRIM33 | PPC |
| H | CCND1 | CDK2 | FAM126A | HLA-DOA | IFNA1 | KISS1R | MINPP1 | PCM1 | RET | TCF7L2 | TSHB | NTC |
* gene selection may be updated based on new research and development
| Catalog No. | GK014 |
|---|---|
| Country of Manufacture | United States |
| Product Code | GQH-TCD |
| Size/Quantity | One 96-well plate, one 20ul reaction per well |
| Product Use | GQH-TCD is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures. |
| Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| Shipping Info | The product is shipped at ambient temperature. Upon receipt, the plate should be stored at 4°C and is good for up to 12 months. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| References | [1] Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F. (2002) "Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes." Genome Biol. 3(7): 1-12. |
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