人细胞凋亡定量PCR芯片试剂盒描述:

ScienCells GeneQuery™ human apoptosis qPCR array kit (GQH-APO) is designed to facilitate gene expression profiling of key genes involved in human apoptosis. 88 genes are selected in this kit based on database and literature research.
GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Apoptosis qPCR Array Plate Layout* (8 controls in Bold and Italic)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
A | ABL1 | BAG1 | BIRC1 | BRAF | CASP6 | DFFA | IL1RAP | MYD88 | PIK3R3 | RELA | TNFRSF9 | β-actin |
B | AIFM1 | BAG3 | BIRC2 | CAPN1 | CASP9 | DIABLO | IL3 | NGF | PPP3CA | RIPK1 | TNFSF10 | GAPDH |
C | AKT1 | BCL10 | BIRC3 | CAPN2 | CD40 | ENDOG | IRAK2 | NOD1 | PPP3CB | RIPK2 | TP53 | LDHA |
D | AKT2 | BCL2 | BIRC5 | CASP1 | CFLAR | FAS | IRAK3 | NOL3 | PPP3R1 | TNF | TP53BP2 | NONO |
E | AKT3 | BCL2L1 | BIRC6 | CASP10 | CHUK | HRK | IRAK4 | PIK3CB | PRKAR1A | TNFRSF10B | TRADD | PPIH |
F | APAF1 | BCL2L10 | BNIP2 | CASP2 | CIDEA | IGF1R | LTBR | PIK3CG | PRKAR2B | TNFRSF10D | TRAF2 | GDC |
G | ATM | BID | BNIP3 | CASP3 | CRADD | IL1B | MAP3K14 | PIK3R1 | PRKX | TNFRSF11B | TRAF3 | PPC |
H | BAD | BIK | BNIP3L | CASP4 | DAPK1 | IL1R1 | MCL1 | PIK3R2 | PYCARD | TNFRSF1B | XIAP | NTC |
* gene selection may be updated based on new research and development
Catalog No. | GK001 |
Country of Manufacture | United States |
Product Code | GQH-APO |
Size/Quantity | One 96-well plate, one 20ul reaction per well |
Product Use | For research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures. |
Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
Shipping Info | Ambient temperature. |
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