pET-30a原核表达载体
英文名称: pET-30a
型号:null    产品货号: 69909-3
价格:请致电:010-57128832,18610462672
品牌: novagen

> δ-促睡眠肽(δSIP)重组蛋白[ PROPERTIES ]
Residues: Met1~Val134 (Accession # Q99576),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 5.3
Predicted Molecular Mass: 18.6kDa
Applications: SDS-PAGE; WB; ELISA; IP.
 δ-促睡眠肽(δSIP)重组蛋白(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
 δ-促睡眠肽(δSIP)重组蛋白[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MNTEMYQTPM EVAVYQLHNF
SISFFSSLLG GDVVSVKLDN SASGASVVAI DNKIEQAMDL VKNHLMYAVR EEVEILKEQI
RELVEKNSQL ERENTLLKTL ASPEQLEKFQ SCLSPEEPAP ESPQVPEAPG GSAV