斑联蛋白(ZYX)重组蛋白

YB235Hu021
Zyxin (ZYX)
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES
4th Edition (Revised in August, 2012)
斑联蛋白(ZYX)重组蛋白[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]
Protein Protein Protein Protein Names: Zyxin
Synonyms Synonyms Synonyms Synonyms: ZYX
Species: Species: Species: Species: Human
Size: 100µg
Source: Escherichia coli-derived
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm. Cytoplasm, cytoskeleton. Nucleus. Cell junction,
focal adhesion.
斑联蛋白(ZYX)重组蛋白[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]
Residues Residues Residues Residues: Cys384~Thr572 (Accession # Q15942), with N-terminal His-Tag.
Grade & Purity: Purity: Purity: Purity: >95%, 22 kDa as determined by SDS-PAGE reducing conditions.
Formulation Formulation Formulation Formulation: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 22.62 kDa
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 6.9
[ PREPARATION PREPARATION PREPARATION PREPARATION ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months . Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was
determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious
degradation and precipitation were observed. (Referring from China Biological Products Standard, which was
calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under
appropriate storage condition.
[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]
斑联蛋白(ZYX)重组蛋白The target protein is fused with N-terminal His-tag, its sequence is listed below.
MGHHHHHHSG SEF-CGRCHQP LARAQPAVRA LGQLFHIACF TCHQCAQQLQ GQQFYSLEGA PYCEGCYTDT LEKCNTCGEP
ITDRMLRATG KAYHPHCFTC VVCARPLEGT SFIVDQANRP HCVPDYHKQY APRCSVCSEP IMPEPGRDET VRVVALDKNF
HMKCYKCEDC GKPLSIEADD NGCFPLDGHV LCRKCHTARA QT
[ REFERENCES REFERENCES REFERENCES REFERENCES ]
1. Macalma T., et al. (1996) J. Biol. Chem. 271:31470-31478. 2. Zumbrunn J., Trueb B. (1996) Eur. J. Biochem. 241:657-663. 3. Hillier L.W., et al. (2003) Nature 424:157-164. 4. Scherer S.W., et al. (2003) Science 300:767-772.