肘臀蛋白(CUBN)重组蛋白

YB411Ra031
Recombinant Cubilin (CUBN)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
[ PROPERTIES ]
肘臀蛋白(CUBN)重组蛋白Source: Prokaryotic expression. Host: E. coli
Residues: Cys3157~Met3274
Tags: N-terminal His-Tag
Homology: Mouse 70%, rat 70%
Tissue Specificity: Placenta, intestine, kidney. Subcellular Location: Endosome membrane; Peripheral membrane protein. Lysosome membrane. Purity: >95%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Traits: Freeze-dried powder
Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01%
sarcosyl and Proclin300. Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification;
Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 5.5
Predicted Molecular Mass: 14.4kDa
肘臀蛋白(CUBN)重组蛋白Accurate Molecular Mass: 14kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
肘臀蛋白(CUBN)重组蛋白observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition. [ SEQUENCE ]
[ IDENTIFICATION ]
Figure 1. SDS-PAGE