TRAF7 Validated CRISPR sgRNA-¾­ÑéÖ¤µÄCRISPR/Cas858»ùÒòÇóýsgRNA
Ó¢ÎÄÃû³Æ£º TRAF7 Validated CRISPR sgRNA
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ɸѡ±ê¼Ç£ºmCherryÏà¹Ø²úÆ·£ºTRAF7 Knockout Cell LineTRAF7 Knockout Cell LysateTRAF7 Knockout Cell Pellets 

ody>¼ÅäÌå(TRAIL)ÖØ×éµ°°×[ PROPERTIES ]
Residues: Thr36~Gly281
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: F1PWB1
Host: E. coli
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 8.6
Predicted Molecular Mass: 32.6kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Ö×Áö»µËÀÒò×ÓÏà¹ØµòÍöÓÕµ¼ÅäÌå(TRAIL)ÖØ×éµ°°× USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
Ö×Áö»µËÀÒò×ÓÏà¹ØµòÍöÓÕµ¼ÅäÌå(TRAIL)ÖØ×éµ°°×[ SEQUENCES ]
The sequence of the target protein is listed below.
TRELK QMQDKYSQSG IACFLKEDDI PWDPSDEESM NNPCWQVKWQ LRQFVRKMIL
KTYEETIPTA PEKQLNIPYV VSDRGSQRVA AHITGTSRRS MFPIPSSKND KALGHKINSW
DSTRKGHSFL NNLHLRNGEL VIHQRGFYYI YSQTYFRFQE PEEIPTGQNR KRNKQMVQYI
YKHTSYPDPI LLMKSARNSC WSKDSEYGLY SIYQGGIFEL KENDRIFVSV SNEQLIDMDQ
EASFFGAFLI G