层粘连蛋白γ3(LAMγ3)重组蛋白

YB084Mu021
Laminin Gamma 3 (LAMC3)
Organism: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
5th Edition (Revised in January, 2013)
层粘连蛋白γ3(LAMγ3)重组蛋白[ DESCRIPTION ]
Protein Names: Laminin Gamma 3
Synonyms: LAMC3
Species: Mouse
Size: 100µg
Source: Escherichia coli-derived
Subcellular Location: Secreted, extracellular space,
extracellular matrix, basement membrane.
[ PROPERTIES ]
Residues: Gly751~Cys875 (Accession # Q9R0B6),
with N-terminal His-Tag.
层粘连蛋白γ3(LAMγ3)重组蛋白Grade & Purity: >95%, 17kDa as determined by
SDS-PAGE reducing conditions.
Formulation: Supplied as lyophilized form in PBS, pH
7.4, containing 5% sucrose, 0.01% sarcosyl.
Endotoxin Level: <1.0 EU per 1µg (determined by the
LAL method).
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Molecular Mass: 14.7kDa
Predicted isoelectric point: 6.2
Mouse LAMC3
[ PREPARATION ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
层粘连蛋白γ3(LAMγ3)重组蛋白Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF- GFYGNAFSGR ADDCQPCPCP GQSACATIPE SGDVVCTHCP
PGQRGRRCES CEDGFFGDPL GLSGAPQPCR RCQCSGNVDL NAVGNCDPHS
GHCLRCLYNT TGAHCEHCRE GFYGSAVATR PVDKC