Introduction: Arl1 is a member of the Arf family of regulatory GTPases, within the Ras superfamily of GTPases (ARFs). ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform.
Physical Appearance (form): Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.
Physical Appearance (form): White or clear
Concentration:1 mg/mL
Storage: -80°C
Preparation Instructions: Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Arl1 Q71L was determined by SDS-PAGE and Coomassie Brilliant Blue Staining
References: 1. Hong, J.-X. et al., J. Biol. Chem. 273: 15872-15876, 1998. 2. Zhang, G.-F. et al., J. Biol. Chem. 270: 21-24, 1995.
