应用：活体组织（in-vivo,in-utero,in-ovo），
       离体组织（ex-vivo），
       贴壁细胞及原代细胞等难以转染细胞的电穿孔（in-vitro）
 
最先进的脉冲技术
                     

 
CUY21EX采用BEX发明的双脉冲和正反交替技术以及目前最先进的指数衰减方波技术：
第一步是高压衰减脉冲电压可达500V，只需一次脉冲即可提供细胞膜高效穿孔；避免了多次脉冲对细胞的损伤
第二步是创新的衰减方波脉冲技术该技术首次实现把衰减指数波脉冲高转化效率和方波的高存活率优势融合为一体，因此CUY21EX具有真正的高转化效率和高存活率
第三步仍然采用反向脉冲技术BEX公司发明的反向脉冲技术在以前的产品应用中，已经验证了反向脉冲可以大大提高转化效率
 
CUY21EX产品技术应用优势举例：
以鸡胚转化为例：                                      
        

上图是采用CUY21EX面市前，其他产品的转化效果，        CUY21EX转化效果图，此图由日本东北大学提供
下图是采用CUY21EX的转化效果，很明显CUY21EX
转化的均一性和效率比其他产品大大提高。此图由名古屋大学提供 

技术指标：
穿孔脉冲
脉冲波形    指数衰减方波脉冲
电压        1 – 500V   1V精度
脉冲长度    0.01 – 99.9msec  0.01V精度
脉冲间隔    0.05 – 99.9 msec  0.01msec精度
脉冲数      1个
 
驱动脉冲
脉冲波形    指数衰减方波脉冲
电压        1 – 500V   1V精度
脉冲长度    0.1 – 99.9msec  0.1V精度
脉冲间隔    1 – 999 msec  1msec精度
脉冲数      1-10个
测量参数
 
电阻测定范围    最大到30Kohm
电压测定范围    1 – 500V
电流测定范围    0.1 – 50A
程序存储        99个
体积重量        W360×L380mm×H180mm,13Kg
 
应用举例;
宫内胚胎转化（In utero）
CUY21凭借极其稳定的脉冲电压和电流检测功能，是目前最适合宫内鼠胚胎和早期鸡胚转化，来自中科院神经所，生物物理所，北京大学，清华大学等用户使用CUY21开展相关工作,文献发表于Nature,Neuron等顶级杂志
Control of cortical GABA circuitry development by Nrg1 and ErbB4 signalling
Fazzari P, Paternain AV, Valiente M, Pla R, Luján R, Lloyd K, Lerma J, Marín O, Rico B
Nature. 2010 Apr 29;464(7293):1376-80.
 
Imaging brain electric signals with genetically targeted voltage-sensitive fluorescent proteins
Nature Methods. 2010 Aug;7(8):643-9. Epub 2010 Jul 11
 
Coordinated changes in dendritic arborization and synaptic strength during neural circuit development
Neuron. 2009 Jan 15;61(1):71-84
 
Lfc and Tctex-1 regulate the genesis of neurons from cortical precursor cells
Nature Neurosci. 2009 Jun;12(6):735-44. Epub 2009 May 17
 
A feedback regulatory loop involving microRNA-9 and nuclear receptor TLX in neural stem cell fate determination
Nature Struct Mol Biol. 2009 Apr;16(4):365-71. Epub 2009 Mar 29
 
Synaptic PRG-1 modulates excitatory transmission via lipid phosphate-mediated signaling
Cell. 2009 Sep 18;138(6):1222-35
 
鸡胚转化(In ovo)
HDAC1 and HDAC2 regulate oligodendrocyte differentiation by disrupting the beta-catenin-TCF interaction
Nat Neurosci. 2009 Jul;12(7):829-38. Epub 2009 Jun 7----------早期鸡胚E2.5和E5.5
 
Integration of growth and specification in chick wing digit-patterning
Nature, Volume 452, Number 7189, Pages 882-886, 17 April 2008
 
成年鼠脑转化(In vivo)
CUY21可提供电流检测，是目前唯一可以进行成年鼠脑转化的仪器，北京大学，清华大学的研究人员已开展相关工作。
Barnabe-Heider et al.
Genetic manipulation of adult mouse neurogenic niches by in vivo electroporation
Nature Methods, Volume 5, Number 2, Pages 189-196, February 2008

新生鼠脑转化（In vivo）
Plexin-A2 and its ligand, Sema6A, control nucleus-centrosome coupling in migrating granule cells
Nature Neuroscience, Volume 11, Number 4, Pages 440-449, April 2008
Microtubule-based nuclear movement occursindependently of centrosome positioning in migrating neurons
PNAS, Volume 104, Number 41, Pages 16182-16187, October 9, 2007
 
鼠视网膜转化
Division and apoptosis of E2f-deficient retinal progenitors
Chen D, Pacal M, Wenzel P, Knoepfler PS, Leone G, Bremner R
Nature. 2009 Dec 17;462(7275):925-9
A role for ligand-gated ion channels in rod photoreceptor development
Neuron, Volume 41, Issue 6, Pages 867-879, 25 March 2004
 
肿瘤基因治疗
Dissociation of EphB2 signaling pathways mediating progenitor cell proliferation and tumor suppression
Cell. 2009 Nov 13;139(4):679-92
 
体外组织/切片转化（Ex-vivo）
鼠
Regenerating cortical connections in a dish: the entorhino-hippocampal organotypic slice co-culture as tool for pharmacological screening of molecules promoting axon regeneration
del Río JA, Soriano E
Nat Protoc. 2010;5(2):217-26. Epub 2010 Jan 21
 
鸡胚胎（体外培养）
Dual mode of paraxial mesoderm formation during chick gastrulation
PNAS, Volume 104, Number 8, Pages 2744-2749, 20 February 2007
 
Collinear activation of Hoxb genes during gastrulation is linked to mesoderm cell ingression
Nature, Volume 442, Number 7102, Pages 568-571, 3 August 2006
 
细胞电穿孔
A2058 melanoma cells and normal human fibroblasts; transfection with FITC-labeled dodecapeptides
BMC Cell Biol. 10, 152009