美国产品商标: neweastbioBrand,null 抗体货号： 21278Item#, Anti-IDH2 Rabbit Polyclonal Antibody<br>英文名称： Anti-IDH2 Rabbit Polyclonal Antibody

IDH2 [21278]
[100 µL]

Catalog Number: 21278

Gene Symbol: IDH2

Description: Anti-IDH2 Rabbit Polyclonal Antibody

Background: Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-132 in IDH to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.

Immunogen: Recombinant protein of human IDH2 (full length).

Applications: ELISA, WB, IHC

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:1000-1:5000
IHC: 1:50-1:500

Concentration: 0.1mg/ml

Host: Rabbit

Format: Liquid

Clonality: Polyclonal

Isotype:

Purity: Purified from serum

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-IDH2 antibody recognizes IDH2 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.

Western blot:

Western blot analysis of recombinant IDH2 protein. Purified His-tagged IDH2 protein (full length) (Cat. #10200) was blotted with anti-IDH2 rabbit polyclonal antibody (Cat. # 21278).

Publications:

1. Specific monoclonal antibodies against IDH1/2 mutations as diagnostic tools for gliomas
Brain Tumor Pathology. January 2015, Volume 32, Issue 1, pp 3-11

2. Mutant IDH inhibits HNF-4α to block hepatocyte differentiation and promote biliary cancer
Nature 513, 110–114 (04 September 2014)

3. Metabolomic comparison between cells overexpressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism
Journal of Neurochemistry. Volume 132, Issue 2, pages 183–193, January 2015