Sephadex G-25medium 葡聚糖凝胶G-25
英文名称： Sephadex G-25medium
型号:null    产品货号： S8141
价格:请致电：010-57128832,18610462672
品牌: 北京市通州区马驹桥联东u谷85a三层

葡聚糖凝胶G-25

品牌：Solarbio | 货号：S8141 | CAS：
  • 英文名称 : Sephadex G-25 medium
  • 储存条件 : 室温
  • 外观（性状） : 白色颗粒
  • 单位 : 瓶
 

 

 

商品货号： 商品品牌： 规格 基本售价： 选择规格
S8141-25g Solarbio 25g 480.00元
S8141-100g Solarbio 100g 1800.00元

 

说明：分离试剂，层析介质.

应    用：利用分子筛作用，进行缓冲液交换、脱盐，分离小分子，去除小分子;工业脱盐。

性    状：白色微球，多孔

凝胶类型：凝胶过滤填料，亲水型凝胶

结构成分：交联***

粒    径： 细颗粒20-80µm，中颗粒50-150µm，粗颗粒100-300µm

工作PH值：2-13

操作温度：4-40℃

球蛋白分离范围：1000-5000

保存条件：4-25℃

 

化学和物理性质:

葡聚糖凝胶是一种珠状的凝胶，含有大量的羟基，很容易在水中和电解质溶液中溶胀。 G型的葡聚糖凝胶有各种不同的交联度，因此它们的溶胀度和分级分离范围也有所不同。 葡聚糖凝胶的溶胀度基本上不因盐和洗涤剂的存在而受影响。

葡聚糖凝胶有不同的粒度。超细级的葡聚糖凝胶是用于需要极高分辨率的柱色谱和薄层色谱。粗级和中级的凝胶用于制备性色谱过程，可在较低的压力下获得较高的流速。 另外，粗级也可用于批量工艺。

化学稳定性:

葡聚糖凝胶不溶于一切溶剂(除非它被化学降解)。 它在水、盐溶液、有机溶剂、碱和弱酸性溶液中都是稳定的，在强酸中凝胶骨架的糖昔键被水解。 长期接触氧化剂将破坏凝胶，因而应避免使用。

物理稳定性:

葡聚糖疑胶并不熔融，可以在湿态、中性PH进行灭菌或在高压灭菌器120℃、30分钟而不影响它的色谱性质。 干态的凝胶加热至120℃以上将开始焦糖化。 葡聚糖凝胶的机械强度取决于交联度。

使用方法:

1. 葡聚糖凝胶预处理：

在室温下，将葡聚糖凝胶干粉浸泡于蒸馏水中至少24小时，并不断搅拌以保证凝胶溶胀，或者用蒸馏水煮沸至少1小时直至充分溶胀，凝胶体积不再变化。

注：在溶胀前，加入蒸馏水搅拌后使其自然沉降，沉降后若上层溶液中漂浮的凝胶碎片颗粒较多，需要重复多次漂洗将其除去，防止层析时阻塞凝胶柱，影响流速。

2. 装柱：

将溶胀好的凝胶根据装柱要求一次性置入柱内，注意保持湿态装柱，并避免柱内产生气泡或断层; 装柱要均匀，可在凝胶耐受的操作压下装柱，装好的凝胶柱可以检测柱效，检测过滤柱装填是否合格。

凝胶层析分离度取决于柱高，为分离不同组分，凝胶柱床必须有适宜的高度：分级分离时，一般需要 100cm 左右长的层析柱，柱高与直径之比为 20:1-100:1，柱高过高时，凝胶挤压变形阻塞会引起较大的反压，应当尽可能避免；分组分离（例如脱盐）时用短柱，柱高控制在40-50cm 以下，柱高与直径的比约为 5:1-10:1。

3. 平衡：

上样前用平衡液平衡层析柱至少3-5个柱体积直到记录仪基线变得平稳为止（例如流出液的PH值等于上柱的Buffer的PH值）；

4. 上样：

分级分离时上样量一般为5%的柱床体积，我们建议初次上样控制在1-2%的床体积，视分离情况可以调整;脱盐时上样量可以达到20%的柱床体积。

样品溶液如有杂质或沉淀应过滤或离心除去，样品的粘度不能过高，否则影响分离效果。

5. 洗脱方法:

可以用无盐水，也可以采用上柱时的缓冲液洗脱；在上柱缓冲液中加入NaCl等梯度洗脱或盐梯度洗脱也可以完全分离纯化；

6. 在位清洗(CIP)

凝胶使用十次后作一次CIP，目的是去除柱床内沉淀的及顽固残留的蛋白。 方法是以40cm/h用1M氢氧化钠反向清洗四个柱体积，再以至少三个柱体积平衡缓冲液再生。

7. 保存

凝胶柱暂时不用，可将其回收，一般方法是将凝胶用水冲洗干净滤干，可加入0.02%叠氮化钠防腐或用20%乙醇浸泡，以控制微生物生长。

北京索莱宝科技有限公司 张净花 010-56371206  13426459985

北京索莱宝供应各种葡聚糖凝胶类产品，欢迎选购

S8120 Sephadex G-10 medium(葡聚糖凝胶G-10) Pharmacia

S8130 Sephadex G-15 medium(葡聚糖凝胶G-15) Pharmacia

S8140 Sephadex G-25 medium(葡聚糖凝胶G-25) Pharmacia

S8150 Sephadex G-50 medium(葡聚糖凝胶G-50) Pharmacia

S8160 Sephadex G-75 medium(葡聚糖凝胶G-75) Pharmacia

S8170 Sephadex G-100 medium(葡聚糖凝胶G-100) Pharmacia

S8111 Sephadex LH-20 (葡聚糖凝胶LH-20） Solarbio

S8110 Sephadex LH-20 (葡聚糖凝胶LH-20） Pharmacia

S9160 Sephadex G-200 medium(葡聚糖凝胶G-200) Solarbio

S9191 Sephadex G-150medium(葡聚糖凝胶G-150) Solarbio

S8121 Sephadex G-10 medium(葡聚糖凝胶G-10) Solarbio

S8131 Sephadex G-15medium（葡聚糖凝胶G-15） Solarbio

S8141 Sephadex G-25medium(葡聚糖凝胶G-25) Solarbio

S8161 Sephadex G-75medium(葡聚糖凝胶G-75) Solarbio

S8151 Sephadex G-50medium(葡聚糖凝胶G-50) Solarbio

S8171 Sephadex G-100medium(葡聚糖凝胶G-100) Solarbio

 

 

 

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