Preparation : | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-1α. Anti-Murine IL-1α specific antibody was purified by affinity chromatography and then biotinylated. |
Catalog #: | 500-P51ABt |
Source : | Rabbit |
Formulation : | A sterile filtered antibody solution was lyophilized from PBS, pH 7.2. |
Stability : | The lyophilized antibody is stable for at least 2 years from date of receipt at -200C. The reconstituted antibody is stable for at least two weeks at 2-80C. Frozen aliquots are stable for at least 6 months when stored at -200C. Avoid repeated freeze-thaw cycles. |
Reconstitution: | Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. |
Applications : | |
Elisa | Direct:To detect mIL-1α by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 C 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 C 0.4 ng/well of recombinant mIL-1α.
Sandwich:To detect mIL-1α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 C 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Murine IL-1α (500-P51A) as a capture antibody, allows the detection of at least 0.2 C 0.4 ng/well of recombinant mIL-1α. |
Western Blot | To detect mIL-1α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Country of Origin : | USA |
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| | Size A | 25μg | $195 |  | | | | Size B | 50μg | $250 | | | | | Size C | 1mg | $4000 | | | |
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| Special Sizes:
Many products can be ordered in custom sizes. Please click here to contact Peprotech with your request. |
| Usage:
For Research Use Only. Not for use in diagnostic or therapeutic procedures. |
