Size  100ug
Cat code  200135
Species Cross-reactivity  H,M
Key Application  WB,IP
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG1
Molecular Weight  63kDa
Sensitivity  This antibody detects endogenous levels of CARM1(C-terminus) and does not cross-react with related proteins.
Immunogen  Purified recombinant human CARM1(C-terminus) protein fragments expressed in E.coli.
UniProt Number  Q86X55
GeneBank ID  NM_199141
Subcellular location  Nucleus. Cytoplasm
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.2% sodium azide,0.1mg/mlBSA and 50% glycerol.
Altername  CARM1;Coactivator associated arginine methyltransferase 1;Histone arginine methyltransferase;Histone-arginine methyltransferase CARM1;PRMT4;Protein arginine N methyltransferase 4.
Gene_symbol  PRMT4
Summary  Protein arginine N-methyltransferases,such as CARM1,catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine.Protein arginine methylation has been implicated in signal transduction,metabolism of nascent pre-RNA,and transcriptional activation.
UniPort summary  Function  Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at ´Arg-17´ (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at ´Arg-2142´, which may loosen its interaction with NCOA2/GRIP1, and at ´Arg-580´ and ´Arg-604´ in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs
Application Images :  
Western blot detection of CARM1(C-terminus) antibody in Hela,A431 & K562 cell lysates using CARM1(C-terminus) antibody (1:200-1:500 diluted).Predicted band size:63KDa.Observed band size:63KDa.
Immunoprecipitation analysis of Hela cell lysates using CARM1 antibody.