Size  100ug
Cat code  200130
Species Cross-reactivity  H,M
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  null
Isotype  IgG2b
Molecular Weight  50kDa
Sensitivity  This antibody detects endogenous levels of SMYD2 and does not cross-react with related proteins.
Immunogen  Purified recombinant human SMYD2 protein fragments expressed in E.coli.
UniProt Number  Q9NRG4
GeneBank ID  NM_020197
Subcellular location  Cytoplasm ? cytosol；Nucleus
Formulation  ascites
Altername  HSKM-B；Histone methyltransferase SMYD2，EC=2.1.1.43；Lysine N-methyltransferase 3C；SET and MYND domain-containing protein 2
Gene_symbol  KMT3C; HSKM-B; ZMYND14
Summary  SET domain-containing proteins, such as SMYD2, catalyze lysine methylation (Brown et al., 2006 [PubMed 16805913]).[supplied by OMIM, Nov 2008]
UniPort summary  Function  Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins. Specifically methylates histone H3 ´Lys-4´ (H3K4me) and dimethylates histone H3 ´Lys-36´ (H3K36me2). Has also methyltransferase activity toward non-histone proteins such as p53/TP53 and RB1. Monomethylates ´Lys-370´ of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates ´Lys-860´ of RB1/RB.
Application Images :  
Western blot detection of SMYD2 in 3T3,HT1080，A431&Jurkat cell lysates and  using SMYD2 antibody (1:1000 diluted). Predicted band size: 50KDa Observed band size: 50KDa