Preparation : | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIG. Anti-Human MIG specific antibody was purified by affinity chromatography employing immobilized hMIG matrix. |
Catalog #: | 500-P50 |
Source : | Rabbits |
Formulation : | A sterile filtered antibody solution was lyophilized from PBS, pH 7.2. |
Stability : | The lyophilized antibody is stable for at least 2 years from date of receipt at -200C. The reconstituted antibody is stable for at least two weeks at 2-80C. Frozen aliquots are stable for at least 6 months when stored at -200C. Avoid repeated freeze-thaw cycles. |
Reconstitution: | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
Applications : | |
Selected References : | Selected List of References Citing This PeproTech Product |
Neutralization | To yield one-half maximal inhibition [ND50] of the biological activity of hMIG (100.0 ng/ml), a concentration of 5.0-7.0 μg/ml of this antibody is required. |
Elisa | Indirect:To detect hMIG by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIG.
Sandwich:To detect hMIG by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human MIG (500-P50Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIG. |
Western Blot | To detect hMIG by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIG is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Country of Origin : | USA |
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| | Size A | 50μg | $195 |  | | | | Size B | 100μg | $250 | | | | | Size C | 1mg | $2100 | | | |
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| Special Sizes:
Many products can be ordered in custom sizes. Please click here to contact Peprotech with your request. |
| Usage:
For Research Use Only. Not for use in diagnostic or therapeutic procedures. |
