GST SpinTrap
英文名称： GST SpinTrap
型号:null    产品货号： 28-9523-59
价格:请致电：010-57128832,18610462672
品牌: 美国

                
            28-4055-00            GST MULTITRAP 4B        
        
            28-4055-01            GST MULTITRAP FF        
        
            28-9523-59            GST SpinTrap        
        
            28-9523-60            GST GraviTrap        
        
            28-9523-61            GST Buffer Kit        
        
            28-9545-49            PGEX-4T-1        
        
            28-9545-50            PGEX-4T-2        
        
            28-9545-52            PGEX-4T-3        
        
            28-9545-53            PGEX-5X-1        
        
            28-9545-54            PGEX-5X-2        
        
            28-9545-55            PGEX-5X-3        
        
            28-9546-46            PGEX-2TK        
        
            28-9546-48            PGEX-6P-1 VECTOR        
        
            28-9546-50            PGEX-6P-2 VECTOR        
        
            28-9546-51            PGEX-6P-3 VECTOR        
        
            28-9546-53            PGEX-2T 25 UG WI/DI        
        
            28-9546-54            PGEX-3X 25 UG WI/DI        
        
            28-9546-56            PGEX-1LT ECOR I/BAP        
        
            11-0033-99            HIS GRAVITRAP        
        
            11-0034-00            HIS BUFFER KIT        
        
            27-4710-01            ANTI-HIS ANTIBODY        
        
            28-4009-89            HIS MULTITRAP HP        
        
            28-4009-90            HIS MULTITRAP FF        
        
            28-4013-53            HIS SPINTRAP        
        
            28-4039-43            COLLECTION PLATE        
        
            28-9321-71            His SpinTrap Kit        
        
            27-1583-01            LIGHT PRIMER MIX        
        
            27-1586-01            HEAVY PRIMER MIX        
        
            27-1588-01            LINKER-PRIMER MIX        
        
            27-1589-01            RS PRIMER MIX        
        
            27-9420-01            ANTI-M13 MONOCLONAL ANTIBODY        
        
            27-9421-01            HRP/ANTI-M13 MONOCLNL CONJUG.        
    
详情请查询以下网址：http://www.gelifesciences.com/webapp/wcs/stores/servlet/productById/zh/GELifeSciences-us/28952359
GST SpinTrap
GST SpinTrap columns are designed for rapid small-scale purification of GST-tagged proteins directly from clarified or unclarified cell lysates
        
  • Preserves protein antigenicity and function.
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  • Yields up to 500 μg of pure protein per column.
    •     
  • Allows direct purification of both clarified and unclarified cell lysates.
    •     
  • Allows direct purification of both clarified and unclarified cell lysates.
 
GST SpinTrap columns are designed for rapid small-scale purification of GST-tagged proteins directly from clarified or unclarified cell lysates. GST-tagged proteins can be recovered under mild elution conditions (10 mM glutathione), which preserves the functionality of the proteins, and more than 90% purity can be obtained in a single step. GST SpinTrap columns are suitable for purification of multiple samples in parallel, for example in screening experiments such as evaluation of chromatography media, growth temperature, culture density, and other variables to find optimal conditions for expression.
 
GST SpinTrap columns are designed for use in a microcentrifuge. Each column contains 50 μl of Glutathione Sepharose 4B, enough for purifying up to 500 μg of recombinant GST and each package contains 50 columns. For increased convenience, GST Buffer Kit contains stock solutions of the buffers needed for purification of GST-tagged proteins.
GST SpinTrap — Technical Specifications
            
            Complete Packsize            50 x 50 µl        
        
            Bed Volume            50 µl        
        
            Binding Capacity/Column            Up to 500 µg recombinant glutathione S-transferase (rGST)        
        
            Storage Conditions            4 to 30°C, PBS and 0,05% Kathon CG/ICP Biocide        
        
            
                                  
        
            Media                     
        
            Ligand            Glutathione        
        
            Average Particle Size            90 μm        
        
            Matrix            Agarose, 4%        
        
            Particle Size            45 µm-165 µm        
        
            Binding Capacity/ml Chromatography Medium            > 25 mg horse liver GST/ml medium1)        
        
            pH stability Working Range            4-13        
        
            pH stability Cleaning            4-13        
        
            Storage Conditions            4 to 30°C, 20% Ethanol        
        
            Chemical Stability            All commonly used aqueous buffers        
            
        1)Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.