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GST SpinTrap

GST SpinTrap columns are designed for rapid small-scale purification of GST-tagged proteins directly from clarified or unclarified cell lysates

Preserves protein antigenicity and function.
Yields up to 500 μg of pure protein per column.
Allows direct purification of both clarified and unclarified cell lysates.
Allows direct purification of both clarified and unclarified cell lysates.

GST SpinTrap columns are designed for rapid small-scale purification of GST-tagged proteins directly from clarified or unclarified cell lysates. GST-tagged proteins can be recovered under mild elution conditions (10 mM glutathione), which preserves the functionality of the proteins, and more than 90% purity can be obtained in a single step. GST SpinTrap columns are suitable for purification of multiple samples in parallel, for example in screening experiments such as evaluation of chromatography media, growth temperature, culture density, and other variables to find optimal conditions for expression.

GST SpinTrap columns are designed for use in a microcentrifuge. Each column contains 50 μl of Glutathione Sepharose 4B, enough for purifying up to 500 μg of recombinant GST and each package contains 50 columns. For increased convenience, GST Buffer Kit contains stock solutions of the buffers needed for purification of GST-tagged proteins.

GST SpinTrap — Technical Specifications

Complete Packsize 50 x 50 µl

Bed Volume 50 µl

Binding Capacity/Column Up to 500 µg recombinant glutathione S-transferase (rGST)

Storage Conditions 4 to 30°C, PBS and 0,05% Kathon CG/ICP Biocide

Media

Ligand Glutathione

Average Particle Size 90 μm

Matrix Agarose, 4%

Particle Size 45 µm-165 µm

Binding Capacity/ml Chromatography Medium > 25 mg horse liver GST/ml medium¹⁾

pH stability Working Range 4-13

pH stability Cleaning 4-13

Storage Conditions 4 to 30°C, 20% Ethanol

Chemical Stability All commonly used aqueous buffers

¹⁾Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.

Complete Packsize	50 x 50 µl
Bed Volume	50 µl
Binding Capacity/Column	Up to 500 µg recombinant glutathione S-transferase (rGST)
Storage Conditions	4 to 30°C, PBS and 0,05% Kathon CG/ICP Biocide

Media

Ligand	Glutathione
Average Particle Size	90 μm
Matrix	Agarose, 4%
Particle Size	45 µm-165 µm
Binding Capacity/ml Chromatography Medium	> 25 mg horse liver GST/ml medium¹⁾
pH stability Working Range	4-13
pH stability Cleaning	4-13
Storage Conditions	4 to 30°C, 20% Ethanol
Chemical Stability	All commonly used aqueous buffers
¹⁾Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.