28-4055-00 GST MULTITRAP 4B

28-4055-01 GST MULTITRAP FF

28-9523-59 GST SpinTrap

28-9523-60 GST GraviTrap

28-9523-61 GST Buffer Kit

28-9545-49 PGEX-4T-1

28-9545-50 PGEX-4T-2

28-9545-52 PGEX-4T-3

28-9545-53 PGEX-5X-1

28-9545-54 PGEX-5X-2

28-9545-55 PGEX-5X-3

28-9546-46 PGEX-2TK

28-9546-48 PGEX-6P-1 VECTOR

28-9546-50 PGEX-6P-2 VECTOR

28-9546-51 PGEX-6P-3 VECTOR

28-9546-53 PGEX-2T 25 UG WI/DI

28-9546-54 PGEX-3X 25 UG WI/DI

28-9546-56 PGEX-1LT ECOR I/BAP

11-0033-99 HIS GRAVITRAP

11-0034-00 HIS BUFFER KIT

27-4710-01 ANTI-HIS ANTIBODY

28-4009-89 HIS MULTITRAP HP

28-4009-90 HIS MULTITRAP FF

28-4013-53 HIS SPINTRAP

28-4039-43 COLLECTION PLATE

28-9321-71 His SpinTrap Kit

27-1583-01 LIGHT PRIMER MIX

27-1586-01 HEAVY PRIMER MIX

27-1588-01 LINKER-PRIMER MIX

27-1589-01 RS PRIMER MIX

27-9420-01 ANTI-M13 MONOCLONAL ANTIBODY

27-9421-01 HRP/ANTI-M13 MONOCLNL CONJUG.

GST MultiTrap FF

GST MultiTrap FF and GST MultiTrap 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and rapid parallel purification of glutathione S-transferase (GST) tagged proteins.

96-well filter plate format for high-throughput screening and parallel purification of GST-tagged* proteins.
Highly reproducible well-to well and plate-to-plate results.
High purity in one step, high chemical stability. Load unclarified samples directly to the wells.
Wells are prepacked with Glutathione Sepharose media for convenience.
Easy and predictable scale-up using prepacked GSTrap FF, GSTPrep FF 16/10, and GSTrap 4B columns.

GST MultiTrap FF and GST MultiTrap 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and purification of glutathione S-transferase (GST) tagged proteins.

Purification of up to 0.5 mg of GST-tagged proteins/well directly from unclarified cell lysate can be achieved using GST MultiTrap filter plates. Direct purification of GST-tagged proteins from unclarified lysate shortens handling time and minimizes degradation of sensitive target proteins. The 96-well plate format gives great flexibility, both when working with automated robotic systems and manually using centrifugation or vacuum. Consistent well-to-well and plate-to-plate performance ensures high reproducibility.

GST MultiTrap FF is prepacked with Glutathione Sepharose 4 fast flow, while GST MultiTrap 4B is prepacked with Glutathione Sepharose 4B.

GST MultiTrap FF — Technical Specifications

Complete Packsize 4 x 96-well filter plates

Storage Conditions 4 - 30°C, 20% Ethanol

Centrifugation Force Recommended 100 x g-500 x g

Centrifugation Force Max. 700 x g

Media

Ligand Glutathione and 10-carbon linker arm

Average Particle Size 90 µm

Matrix Highly cross-linked 4% agarose

Binding Capacity/ml Chromatography Medium ≈ 10 mg recombinant GST/ml medium¹⁾

pH stability Working Range 3-12

pH stability Cleaning 3-12

Pressure/Flow Specification base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

Flow Velocity <450 cm/h

Ligand Coupling Method Epoxy activation

Storage Conditions 4 to 30°C, 20% Ethanol

Chemical Stability All commonly used aqueous buffers, e.g. 1 M acetate, pH 6.0, 0.1 M NaOH, 70% ethanol or 6 M guanidine-hydrochloride for 1 h at room temperature

¹⁾Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.

Column

Complete Packsize 4 x 96-well filter plates

Number of Wells 96

Sample Volume 600 μl/well

Reproducibility Between Wells ± 10% in yield

Material Polypropylene (PP) and polyethylene (PE).

Dimensions 127.8 x 85.5 x 30.6 mm