17-6001-78
            
IPG BUFFER, PH 6-11
        
    
        
        
            
17-6002-02
            
IPG BUFFER, PH 3-5.6 NL
        
        
            
17-6002-04
            
IPG BUFFER, PH 4.5-5.5
        
        
            
17-6002-05
            
IPG BUFFER, PH 5.0-6.0
        
        
            
17-6002-06
            
IPG BUFFER, PH 5.3-6.5
        
    
IPG Buffers
Technical Information

 
IPG Buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobiline™ DryStrip gels for isoelectric focusing, run as the first dimension of 2-D electrophoresis or as a separate application. IPG Buffer pH 3-10 and pH 3-10 NL have been improved with a new formula to increase resolution, sensitivity, and spot intensity.
 
Each IPG Buffer produces a more uniform conductivity along the Immobiline DryStrip during focusing, resulting in wider latitude in run times. IPG Buffers also eliminate high background staining. IPG Buffers are supplied in 1 ml aliquots of water. The buffer is diluted 50- or 200-fold in the rehydration solution, depending on the first-dimension system and pH range of the strip.
 
    
        
            
TECHNICAL SPECIFICATIONS
        
        
            
Content 
            
Specialized carrier ampholytes in aqueous solution 
        
        
            
Volume 
            
1 ml 
        
        
            
Storage 
            
4°C to 8°C 
        
        
            
Shelf life 
            
36 months 
        
    
 
 
The basic area of 2-D protein spot maps using Immobiline™ DryStrip pH 3-10 NL (18 cm) together with A) improved IPG Buffer pH 3-10 NL or B) the previous recipe for IPG Buffer pH 3-10 NL. The improved IPG Buffer formula results in additional spots, and some of the differences are indicated in the figure.
http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=165728