预优化siRNA转染试剂-Smooth Muscle
英文名称： GenMute™ siRNA Transfection Reagent for Smooth Muscle Cells
型号:null    产品货号： SL100568-SMC
价格:请致电：010-57128832,18610462672
品牌: 美国

Description:
GenMute™ siRNA Transfection Reagent for SMC is pre-optimized for transfecting siRNA to smooth muscle cells (SMC). 

Refer to the following optimal transfection conditions for maximal silencing on SMC. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Cell culture medium
Confluency of cell on the day of transfection
Optimal siRNA concentration
Diluent for siRNA and GenMute™ Reagent
Incubation Time to Form GenMute™/siRNA Complex
Maximal Silencing Efficiency 

Transfection Results:
Reporter Gene
Silencing Efficiency (% )


DMEM with 4.5 g/L glucose, 10% FBS
~60%
40 nM
1xGenMute™ Transfection Buffer
~15 minutes at RT
48 hours


Renilla Luciferase
85%


Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

Data Sheet & Protocol.pdf