产品特点：
英文名称： Cell-Based Phosphotyrosine ELISA kit
RayBio Cell-Based Phosphotyrosine ELISA kit,是快速、便捷、灵敏度极高的ELISA试剂盒，广泛应用于检测细胞中重要生物通路蛋白的活性及功能。它可用于检测***酸化蛋白的相对含量或各种处理、抑制、激活等条件刺激对培养着的活细胞的影响，样本可以是来自人、大鼠、兔等。
工作原理：
试剂盒组分： 实验步骤： 产品订购：使用常规的酶标仪读取结果
1. Microplate (Item A): two of 96 well tissue culture plates (12 x 8 wells) for cell culture.
2. Wash Buffer Concentrate A (20x) (Item B): 30 ml of 20x concentrated buffer.
3. Wash Buffer Concentrate B (20x) (Item C): 30 ml of 20x concentrated buffer.
4. Fixing Solution (Item D): 30 ml of fixing solution.
5. Quenching Buffer Concentrate (Item E): 2 ml of 30x concentrated solution.
6. Blocking Solution (5x) (Item F): 20 ml of 5x concentrated solution.
7. Mouse Anti-Phospho-Stat1 (Tyr701) Concentrate (Item G1): one tube (30 assays) of anti-phospho-Stat1 (Thy701).
8. Mouse Anti-Stat1 Concentrate (Item H1): one tube (30 assays) of anti-Stat1.
9. Mouse Anti-Phospho-Stat3 (Tyr705) Concentrate (Item G2): one tube (30 assays) of anti-phospho-Stat3 (Tyr705).
10. Mouse Anti-Stat3 Concentrate (Item H2): one tube (30 assays) of anti-Stat3.
11. Mouse Anti-Phospho-Stat5 (Tyr694) Concentrate (Item G3): one tube (30 assays) of anti-phospho-Stat5 (Tyr694).
12. Mouse Anti-Stat5 Concentrate (Item H3): one tube (30 assays) of anti-Stat5.
13. Anti-Mouse IgG Concentrate (Item I): two tubes of 10 μl concentrated HRP-conjugated anti-mouse IgG.
14. TMB One-Step Substrate Reagent (Item J): two bottles, 12 ml of 3,3’,5,5’-tetramethylbenzidine (TMB) in buffered solution.
15. Stop Solution (Item K): 14 ml of sulfuric acid.
1. Add cells
2. Treatment with stimulators or inhibitors
3. Fixing and blocking
4. Anti-phospho-protein antibody or anti-pan-protein antibody
5. HRP-conjugated secondary antibody
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