英文名称： DIG High Prime DNA Labeling and Detection Starter Kit 1：Kit for color detection with NBT/BCIP
| DIG高效DNA标记及检测Starter Kit 1 | |||
| DIG High Prime DNA Labeling and Detection Starter Kit 1：Kit for color detection with NBT/BCIP | |||
| Roche公司现为用户提供一种新的DIG标记及检测试盒DIG High Prime DNA Labeling and Detection Kit I。与原有的DIG DNA Labeling and Detection kit(Cat.No.1093657)比较，本产品有以下特点： ●能对10×10cm印迹膜作12个标记反应及24个显色检测反应，是DIG DNA Labeling and Detection Kit(Cat.No.1093657)份量的一半，所以特别适合初用者及需处理较少量样品的用户使用。 ●试剂盒采用的是DIG高效标记试剂(Cat.No.1585606)，其标记效率是标准DIG随机引物标记法的3-10倍，而且预混了标记需要的所有试剂，即开即用，令操作更简单和方便。 ●试剂盒中还包括了DIG定量/对照试纸条，令您能在30分钟内只用小量试剂便可估计DIG标记探针的浓度，方便您取得理想的信噪比。 ●用于显色的NBT/BCIP底物为预混的浓缩度，而封闭液则以10倍浓度形式供应，简化了操作程序。 | |||
| 产品号 | 产品规格 | 厂商 | |
| 1745832 11745832910 | Kit：1 kit (12 labeling reactions and 24 detection reactions) | Roche | |
DIG高效DNA标记及检测StarterKit 1 说明书
DIG-High Prime DNA Labeling and Detection Starter Kit I
Application
DIG-High Prime labeled probes can be used in Southern-, northern- and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and color detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering a ready-made blocking solution, combined stock solution of NBT/ BCIP, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.
Benefits
The High Prime method of labeling offers many benefits. Plasmid, cosmid, and lambda phage DNA have all been used as templates. We have achieved equal efficiency when labeling 10 ng to 3 µg of template DNA, which may be linear or supercoiled.
Product Description
Sensitivity and specificity: The standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 hour of labeling, and 2.3 µg labeled DNA after a 20-hour reaction. In a dot-blot hybridization, 0.1 pg of homologous DNA is detectable after a 16-hour color development (probe concentrations at 20 ng/ml). A single-copy human gene (tPA) is detectable in a Southern blot, loading 1 µg of digested placenta DNA.
Contents
1. DIG-High Prime, 5x conc., 50 µl
2. DIG-labeled Control DNA, 20 µl, (5 µg/ml), pBR328 (linearized with Bam HI)
3. DNA Dilution Buffer, 3 x 1 ml
4. Anti-digoxigenin-AP Conjugate, 100 µl
5. NBT/BCIP Stock Solution, conc., 6 x 1 ml
6. Blocking Solution, 10x conc., 4 x 100 ml
DIG Easy Hyb Granules, 4 x 100 ml