官方网址：http://www.sinobiological.cn/histone-cluster-1-cDNA-Clone-g-648.html
 General Information 
Manufactured By:  Sino Biological Inc. 

Gene Bank Ref.ID: AL109948.9 (69913-70323)

cDNA Size: 411 

Synonym: MGC126031; HIST2H3PS2

Source:  Human.
Description 
cDNA Description:  ORF Clone of Homo sapiens histone cluster 2, H3, pseudogene 2 DNA

Vector:  Commercial pMD vector.(Produced by TAKARA. 
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for three point mutations:396C/T, 399G/A and 408C/T not causing the amino acid variation.
Shipping
5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature. 
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
Usage Protocol
    
Remove the marked edge in the center of the spot .
    
Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
    
Transfer the tube to a heating block at 95oC for 15–30 min .
    
At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap, the sample approximately 60 times.
    
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
cDNA Sequence and Quality Control 
基因序列:

 1 ATGGCCCGTA CTAAGCAGAC TGCCCGCAAG TCGACCGGCG GCAAGGCCCC GAGGAAGCAG 61 CTGGCTACCA AAGCGGCCCG CAAGAGCGCG CCGGCCACGG GCGGGGTGAA GAAGCCGCAC 121 CGCTACCGGC CCGGCACCGT GGCTCTGCGG GAGATCCGGC GCTATCAGAA GTCTACGGAG 181 CTGCTGATCC GCAAGCTGCC CTTCCAGCGG CTGGTACGCG AGATCGCGCA GGAGTTTAAG 241 ACGGACCTGC GCTTCCAGAG CTCGGCCGTG ATGGCGCTGC AGGAGGCCAG AGAGGCCTAC 301 CTGGTGGGGC TGTTCGAAGA CACGAACCTG TGCGCCATCC ATGCCAAGCG CGTGACCATC 361 ATGCCCAAGG ACATCCAGTT GGTCAGCCGC ATCCGTGGAG AGCGGGCTTA A

Quality Control:   cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).
 
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