英文名称： HITRAP IMAC HP
| 17-0920-03 | HITRAP IMAC HP, 5 X 1 ML |
| 17-0920-05 | HITRAP IMAC HP, 5 X 5 ML |
| 17-0921-02 | HITRAP IMAC FF, 5 X 1ML |
| 17-0921-04 | HITRAP IMAC FF, 5 X 5 ML |
| 17-0978-13 | HITRAP BUTYL-S FF, 5 X 1ML |
| 17-0978-14 | HITRAP BUTYL-S FF, 5 X 5ML |
| 17-1128-01 | MABTRAP KIT |
| 17-1151-01 | HITRAP SP HP, 5 X 1 ML |
| 17-1152-01 | HITRAP SP HP, 5X5 ML |
| 17-1153-01 | HITRAP Q HP, 5 X 1 ML |
| 17-1154-01 | HITRAP Q HP 5X5 ML |
| 17-1351-01 | HITRAP PHENYL HP, 5 X 1ML |
| 17-1353-01 | HITRAP PHENYL FF (LS), 5X1ML |
| 17-1355-01 | HITRAP PHENYL FF (HS),5 X 1ML |
| 17-1357-01 | HITRAP BUTYL FF, 5 X 1 ML |
| 17-1359-01 | HITRAP OCTYL FF, 5 X 1 ML |
| 17-1408-01 | HITRAP DESALTING, 5X5ML |
| 17-5053-01 | HITRAP Q FF, 5 X 1 ML |
| 17-5054-01 | HITRAP SP FF, 5 X 1 ML |
| 17-5055-01 | HITRAP DEAE FF 5 X 1 ML |
| 17-5056-01 | HITRAP CM FF, 5 X 1 ML |
IMAC Sepharose High Performance Media/HiTrap IMAC HP Columns
- For optimizing purification of histidine-tagged* proteins when Ni2+ is not the best choice of metal ion.
- Conveniently charge with your metal of choice.
- Small bead size for high performance purification.
- High binding capacity.
- Prepacked HiTrap columns for convenient purification.
IMAC Sepharose High Performance Media/HiTrap IMAC HP Columns
Technical Information
<sub>6</sub> from <italic>E. coli</italic> lysate using HiTrap IMAC HP column charged with different metal ions.'))
* For licensing information, see Legal Info.
| TECHNICAL SPECIFICATIONS | |
| Matrix | Highly cross-linked agarose, 6% |
| Average particle size | 34 μm |
| Binding capacity‡ | At least 40 mg histidine-tagged protein/ml medium when charged with Ni2+ |
| Compatibility | Stable in all commonly used buffers |
| Chemical stability* | For one week at 40°C: 0.01 M HCl, 0.1 M NaOH For 12 h: 1M NaOH, 70% acetic acid 30 min tested 30% 2-propanol 1 h tested 2% SDS |
| pH stability* | 3-12 (long term), 2-14 (short term) |
| HiTrap™ prepacked columns | |
| Column volume | 1 ml and 5 ml |
| Column dimensions | 0.7 × 2.5 cm (1 ml), 1.6 × 2.5 cm (5 ml) |
| Recommended flow rate† | 1 ml/min (1 ml), 5 ml/min (5 ml) |
| Max. pressure† | 3 bar (0.3 MPa, 42 psi) |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
| * Medium uncharged with metal ions. † H2O at room temperature. ‡ Protein- and metal-ion dependent.
| |
<sub>6</sub> from <italic>E. coli</italic> lysate using HiTrap IMAC HP column charged with different metal ions.'))
Purification of GFP-(His)6 from E. coli lysate using HiTrap IMAC HP column charged with different metal ions.
<sub>6</sub> fractions purified with different metal ions according to the chromatogram above. '))
SDS-PAGE of GFP-(His)6 fractions purified with different metal ions according to the chromatogram above
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