17-5073-01 | SP SEPHAROSE XL, 300 ML | 17-5316-02 | Capto Q 100 ml | 17-5316-10 | CAPTO Q, 25 ML | 17-5317-02 | Capto MMC 100ml | 17-5317-10 | CAPTO MMC, 25ML | 17-5432-01 | Butyl Sepharose HP, 25 ml | 17-5432-02 | Butyl Sepharose HP, 200ml | 17-5437-01 | Q SEPHAROSE XL VIRUS LIC 300ML | 17-5437-10 | Q SEPHAROSE XL VIRUS LIC 25 ML | 17-5441-01 | CAPTO S, 100 ML | 17-5441-10 | CAPTO S, 25 ML | 17-5443-01 | CAPTO DEAE, 100 ML | 17-5443-10 | CAPTO DEAE, 25 ML | 17-5444-10 | CAPTO adhere 25 ML | HiPrep™ IEX XL Columns and IEX Sepharose™ XL Lab Packs - Prepacked with Q Sepharose™ XL* or SP Sepharose XL media, 20-ml preparative anion- and cation-exchange columns provide high loading capacity and fast purifications.
- Optimized for reliable, reproducible separations.
- Q Sepharose XL virus licensed is specifically intended for the purification of viruses.
- Compatible with simple pump-based configurations and other chromatography systems such as ÄKTA™design
HiPrep™ IEX XL Columns and IEX Sepharose™ XL Lab PacksTechnical Information
HiPrep™ Q XL 16-10 and HiPrep™ SP XL 16/10 combine high binding capacity with high throughput.
* Licensing information Separating viral particles with "Q Sepharose™ XL" products may require a license under United States patent 6,537,793 B2 and foreign equivalents owned by Gencell SAS. Such a license is not included with the purchase of Q Sepharose™ XL but is included with the purchase of "Q Sepharose™ XL virus licensed" products. With the purchase of "Q Sepharose XL virus licensed" the customer is granted a free limited license under US patent 6,537,793 B2 and foreign equivalents owned by Gencell SAS to separate viral particles solely through use of the product purchased.
TECHNICAL SPECIFICATIONS | Ion exchanger type | | Q Sepharose™ XL | Quaternary amino strong anion exchanger | SP Sepharose™ XL | Sulfopropyl strong cation exchanger | Ionic capacity | | Q Sepharose™ XL | 0.18-0.26 mmol Cl-/ml | SP Sepharose™ XL | 0.18-0.25 mmol H+/ml | Dynamic capacity | | Q Sepharose™ XL | > 130 mg BSA/ml medium | SP Sepharose™ XL | > 160 mg lysozyme/ml medium | Matrix | Dextran attached to 6% highly cross-linked spherical agarose | Average particle size | 90 μm | pH stability | | Q Sepharose™ XL | 2-14 (short term), 2-12 (long term) | SP Sepharose™ XL | 3-14 (short term), 4-13 (long term) | Chemical stability | All commonly used buffers, nonionic detergents, 1 M NaOH, 6 M guanidine hydrochloride | Avoid using | | Q Sepharose™ XL | Oxidizing agents, anionic detergents, and buffers | SP Sepharose™ XL | Oxidizing agents, cationic detergents, and buffers | Storage | 20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (SP) | Storage temperature | 4°C to 30°C | Prepacked HiPrep™ 16/10 Columns | Column volume | 20 ml | Column dimensions | 1.6 × 10 cm | Recommended flow rate* | 2-10 ml/min (60-300 cm/h) | Max. flow rate* | 10 ml/min (300 cm/h) | Max. pressure over packed bed during operation* | 1.5 bar (22 psi, 0.15 MPa) | Column hardware pressure limit | 5 bar (73 psi, 0.5 MPa) | Storage | 20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (SP) | Storage temperature | 4°C to 30°C | * H2O at 25°C. | Partial purification of the recombinant protein deacetoxycephalosporin C synthase (DAOCS) expressed in E. coli on HiPrep™ Q XL 16/10.
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