NHS Mag Sepharose
NHS Mag Sepharose are magnetic beads designed for pull-down techniques enabling rapid capture and enrichment of selected proteins based on affinity
- Visible and dense Sepharose based magnetic beads with protein A ligand, easy to spot and collect bound target protein
- Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
- Simple capture of target protein in small or large sample volumes (low microliter to high milliliter scale)
- Optimized capacity for enrichment/immunoprecipitation of target proteins - only low amounts of antibody needed
- Flexible protocols with elution conditions optimized for both electrophoresis and MS analysis
NHS Mag Sepharose are magnetic beads designed for pull-down techniques enabling rapid capture and enrichment of selected proteins based on affinity. An antibody or other protein with desired affinity is covalently coupled to the medium via primary amines for the purpose of capture of target proteins of interest
NHS Mag Sepharose — Technical Specifications
Application | Designed for covalent coupling of any protein or biomolecule containing a free amine group and is used for various types of pull-down experiments Used for biomarker reseach |
Matrix | Paramagnetic, spherical, highly cross-linked agarose particles |
Ligand | N-hydroxysuccinimide |
Binding Capacity | 8 µmol/ml gel - 14 µmol/ml gel |
Operating Temp. | Room temperature |
Storage Conditions | 4 - 8°C, 2-propanol |
Stability | Stable |