17-1408-01	HITRAP DESALTING, 5X5ML
17-5053-01	HITRAP Q FF, 5 X 1 ML
17-5054-01	HITRAP SP FF, 5 X 1 ML
17-5055-01	HITRAP DEAE FF 5 X 1 ML
17-5056-01	HITRAP CM FF, 5 X 1 ML
17-5079-01	HITRAP RPROTEIN A FF, 5X1ML
17-5079-02	HITRAP RPROTEIN A FF, 2 X 1 ML
17-5080-01	HITRAP RPROTEIN A FF, 1X5ML
17-5080-02	HITRAP RPROTEIN A FF, 5 X 5 ML
17-5110-01	HITRAP IGM PURIFICATION 5X1ML
17-5111-01	HITRAP IGY PURIFICATION 1X5 ML
17-5112-01	HITRAP STREPTAVIDIN HP5X1 ML
17-5130-01	GSTRAP FF, 5X1ML
17-5130-02	GSTRAP FF, 2X1ML
17-5130-05	GSTRAP FF, 100 X 1 ML
17-5131-01	GSTRAP FF, 1X5ML
17-5131-02	GSTRAP FF 5 X 5 ML
17-5131-05	GSTRAP FF, 100 X 5 ML
17-5143-01	HITRAP BENZAMIDINE FF 5 X 1ML
17-5143-02	HITRAP BENZAMIDINE FF 2 X 1 ML
17-5144-01	HITRAP BENZAMIDINE FF 1 X 5 ML
17-5154-01	HITRAP DEAE FF 5 X 5 ML

HiTrap™ Desalting Column

• Prepacked with Sephadex™ G-25 Superfine, the ready to use 5 ml HiTrap™ column is an excellent choice for fast, convenient desalting and buffer exchange using either a pump or a syringe.
• Replaces dialysis as the method of choice for desalting.
• Handles sample volume of 1.5 ml, or larger volumes by connecting up to five columns in series.
• Desalting requires less than one minute due to the high rigidity of Sephadex™ G-25 and the optimized packing technology.
• Connectors are included for easy connection to any chromatography system.

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HiTrap™ Desalting Column

Technical Information

HiTrap™ Desalting allows efficient, easy-to-perform group separations with a syringe or pump.

 

TECHNICAL SPECIFICATIONS
HiTrap™ Desalting Column
Column volume	5 ml
Medium	Sephadex™ G-25 Superfine
Column dimensions	1.6 × 2.5 cm
Void volume	1.5 ml
Sample volume	0.25-1.5 ml
Elution volume	1.0-2.0 ml
Exclusion limit (Mr)	5 × 103 globular protein
Max. flow rate (H2O at 25°C)	15 ml/min
Recommended flow rate	1-10 ml/min
Max. pressure	3 bar (43 psi, 0.3 MPa)
Chemical stability	Stable in all commonly used buffer systems
pH stability	2-13 (long and short term)
Storage	20% ethanol
Storage temperature	4°C to 30°C

 

Highly efficient desalting in 30 s using HiTrap™ Desalting Column.

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