17-1128-01	MABTRAP KIT
17-1151-01	HITRAP SP HP, 5 X 1 ML
17-1152-01	HITRAP SP HP, 5X5 ML
17-1153-01	HITRAP Q HP, 5 X 1 ML
17-1154-01	HITRAP Q HP 5X5 ML
17-1351-01	HITRAP PHENYL HP, 5 X 1ML
17-1353-01	HITRAP PHENYL FF (LS), 5X1ML
17-1355-01	HITRAP PHENYL FF (HS),5 X 1ML
17-1357-01	HITRAP BUTYL FF, 5 X 1 ML
17-1359-01	HITRAP OCTYL FF, 5 X 1 ML
17-1408-01	HITRAP DESALTING, 5X5ML
17-5053-01	HITRAP Q FF, 5 X 1 ML
17-5054-01	HITRAP SP FF, 5 X 1 ML
17-5055-01	HITRAP DEAE FF 5 X 1 ML
17-5056-01	HITRAP CM FF, 5 X 1 ML
17-5079-01	HITRAP RPROTEIN A FF, 5X1ML
17-5079-02	HITRAP RPROTEIN A FF, 2 X 1 ML
17-5080-01	HITRAP RPROTEIN A FF, 1X5ML
17-5080-02	HITRAP RPROTEIN A FF, 5 X 5 ML
17-5110-01	HITRAP IGM PURIFICATION 5X1ML
17-5111-01	HITRAP IGY PURIFICATION 1X5 ML
17-5112-01	HITRAP STREPTAVIDIN HP5X1 ML

MAbTrap™ Kit

• Convenient kit for purification of IgG from monoclonal and polyclonal material in less than 15 min, using a syringe.
• Kit contains one HiTrap™ Protein G HP column prepacked with Protein G Sepharose™ High Performance and enough buffer concentrate for 20 preparations with a syringe.

MAbTrap™ Kit

Technical Information

The kit includes: HiTrap™ Protein G HP (1-ml column), binding buffer (50 ml), elution buffer (15 ml), neutralizing buffer (25 ml), Luer connector, stop plugs female (1/16"), syringe, and instructions.

 

TECHNICAL SPECIFICATIONS
Bed volume	1 ml
Ligand	Recombinant protein G lacking albumin-binding region
No. of IgG binding sites per ligand	2
Degree of substitution	» 2 mg protein G/ml medium
Matrix	Highly cross-linked agarose, 6%
Binding capacity	> 25 mg human IgG/ml medium
Maximum flow rate	4 ml/min (» 2 drops/s)
Chemical stability	All commonly used aqueous buffers
pH stability	3-9 (long term), 2*-9 (short term)
Storage	20% ethanol
Storage temperature	4°C to 8°C
* pH below 3 is sometimes required to elute strongly bound IgG species. However,  protein ligands may hydrolyze at very low pH.

 

Purification of mouse IgG₁ from cell culture supernatant using MAbTrap™ Kit and a syringe.