17-0671-01 | MONO Q PC 1.6/5 | 17-0672-01 | MONO S PC 1.6/5 | 17-0673-01 | SUPEROSE 6 PC 3.2/30 | 17-0674-01 | SUPEROSE 12 PC 3.2/30 | 17-0686-01 | MINI Q PC 3.2/3 | 17-0687-01 | MINI S PC 3.2/3 | 17-0771-01 | SUPERDEX 75 PC 3.2/30 | 17-1089-01 | SUPERDEX 200 PC 3.2/30 | 17-1455-01 | PRECISION COLUMN HOLDER | 17-1458-01 | SUPERDEX PEPTIDE PC 3.2/30 | 17-1550-01 | COLUMN HOLDER, 10 CM | 18-1015-28 | PROTECTIVE CAPS (2/PK) | 17-5057-01 | MICRORPC C2-C18 ST 4,6-100 | 17-5068-01 | SOURCE 15RPC ST 4.6/100 | 17-5116-01 | SOURCE 5RPC ST 4,6-150 | 17-5172-01 | SUPEROSE 6 10/300 GL | 17-5173-01 | SUPEROSE 12 10/300 GL | 17-5174-01 | SUPERDEX 75 10/300 GL | 17-5175-01 | SUPERDEX 200 10/300 GL | 17-5176-01 | SUPERDEX PEPTIDE 10/300 GL | 28-9065-61 | Superdex 200 5_150 GL | 28-9205-04 | Superdex 75 5_150 GL | 17-0506-01 | PP COLUMN MONO Q HR 16/10 | 17-0507-01 | PP COLUMN MONO S HR 16/10 | MiniBeads™ Columns - Innovative,monodisperse 3-μm nonporous beads yield exceptional resolution, speed, reproducibility, and durability with somewhat lower capacity than MonoBeads™.
- Tricorn™ high-performance columns (4.6/50 PE) prepacked with MiniBeads™ are optimized for use with ÄKTAdesign and comparable LC systems.
- Nonporous matrix of MiniBeads™ improves resolution and flow kinetics, better withstands back pressure, and minimizes nonspecific adsorption of the sample.
- Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q and S ion exchangers.
- PC columns are designed for micropurification.
MiniBeads™ ColumnsTechnical Information
MiniBeads ion exchange media are available prepacked in Tricorn and PC columns for high-performance, high-resolution purification of proteins, peptides, and polynucleotides.
TECHNICAL SPECIFICATIONS | MiniBeads™ | Ion exchanger type | Mini Q™ | Quaternary ammonium strong anion | Mini S™ | Methyl sulfonate strong cation | Ionic capacity | | Mini Q™ | 85-115 μmol Cl-/ml packed medium | Mini S™ | 13-29 μmol H+/ml packed medium | pH stability | 3-11 (working), 2-14 (short term) | Chemical stability | Stable in all common buffers except high concentration oxidizing and reactive agents, DMSO, DMF, and formic acid. | Base matrix | Monodisperse polystyrene/divinyl benzene particles | Particle size | 3 μm | Storage | 20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (S) | Storage temperature | 4°C to 30°C | MiniBeads™ Columns* | Bed volume | | PC 3.2/3 | 0.24 ml | 4.6/50 PE | 0.8 ml | Max. pressure | | PC 3.2/3 | 100 bar (1450 psi, 10 MPa) | 4.6/50 PE | 180 bar (2610 psi, 18 MPa) | Recommended flow rate | | PC 3.2/3 | 0.1-1.0 ml/min | 4.6/50 PE | 0.5-2.0 ml/min | * Columns not suitable for use with ÄKTAprime™ plus system. | The exceptional resolution with Mini S 4.6/50 PE makes it a powerful tool for purity check of synthetic oligonucleotides
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