| TECHNICAL SPECIFICATIONS |
| | nProtein A Sepharose 4 Fast Flow | Protein G Sepharose 4 Fast Flow |
| Ligand | Native protein A | Recombinant protein G lacking albumin-binding region |
| Ligand density | » 6 mg Protein A/ml | » 2 mg Protein G/ml |
| Ligand coupling method | Cyanogen bromide activation | Cyanogen bromide activation |
| Matrix | Highly cross-linked agarose, 4% | Highly cross-linked agarose, 4% |
| Binding capacity | > 30 mg human IgG/ml medium | > 20 mg human IgG/ml medium |
| pH stability | 3 to 9 (working) | 3 to 9 (working) |
| Storage | 20% ethanol | 20% ethanol |
| Storage temperature | 4°C to 8°C | 4°C to 8°C |