Yeast Protein Extraction Buffer Kit

 

Yeast Protein Extraction Buffer Kit is a mild detergent-based cell lysis method developed for total extraction of soluble proteins from yeast cells. This kit eliminates the need for glass beads, a common method of mechanical lysis for yeast cells, and is a proprietary improvement on the Zymolyase based method for spheroplast preparation and extraction of soluble proteins from yeast cells. After cell lysis, Yeast Protein Extraction Buffer is added to the yeast pellets, referred to as spheroplasts, to extract biologically active proteins. The kit is compatible with any downstream application including enzymes assays, chromatography procedures, and gel electrophoresis applications, and is suitable for preparation of approximately 10 ml yeast cell pellets suspension as single or as multiple smaller preps.

Yeast Protein Extraction Buffer Kit — Technical Specifications

devices while the visibility of the beads ensures reliable collection of all bound target peptides

 

Together with MagRack 6, a separation tool for handling the beads in microcentrifuge tubes, up to six samples can be processed in parallel

TiO₂ Mag Sepharose — Technical Specifications

Complete Packsize

Application	Used for selective capture and enrichment of phosphopeptides Used for biomarker reseach
Matrix	Paramagnetic, spherical, highly cross-linked agarose particles
Ligand	TiO2
Operating Temp.	Room temperature
Storage Conditions	4 - 30°C, 20% Ethanol
Stability	Stable