17-5132-02	GLUTATHIONE SEPH 4 FF, 100 ML
17-5132-03	GLUTATHIONE SEPH 4 FF, 500 ML
17-5138-01	RMP PROT A SEPH FF 5 ML
17-5138-02	RMP PROT A SEPH FF 25 ML
17-5138-03	RMP PROT A SEP FF 200 ML
17-5199-01	MABSELECT 25 ML
17-5199-02	MABSELECT 200 ML
17-5268-01	NI SEPHAROSE HP 25 ML
17-5268-02	NI SEPHAROSE HP 100 ML
17-5269-02	MABSELECT XTRA 200 ML
17-5269-07	MABSELECT XTRA 25ML
17-5279-01	GLUTATHIONE SEPHAROSE HP 25 ML
17-5279-02	GLUTATHIONE SEPH HP 100 ML
17-5280-01	NPROTEIN A SEPHAROSE 4 FF 5ML
17-5280-02	NPROTEIN A SEPH 4 FF 200 ML
17-5280-04	NPROTEIN A SEPHAROSE 4FF 25ML
17-5318-01	NI SEPHAROSE 6FF, 25 ML
17-5318-02	NI SEPHAROSE 6FF, 100 ML
17-5318-03	NI SEPHAROSE 6FF, 500 ML
17-5318-06	NI SEPHAROSE 6 FF 5 ML
17-5438-01	MABSELECT SURE 25 ML
17-5438-02	MABSELECT SURE 200 ML
17-5448-01	Capto Blue 25 ml
17-5474-01	MabSelect SuRe LX 25 ML
17-5474-02	MabSelect SuRe LX 200 ML
17-6002-35	IMMUNOPRECIP STARTER PACK
18-1022-29	HB AFFINITY CHROMATOGRAPHY
18-1037-46	ANTIBODY PURIFICATION HB
28-9355-97	Dextrin Sepharose High Perform
28-9355-98	Dextrin Sepharose High Perform
28-9356-00	StrepTactin Sepharose High Per
28-9805-82	MembraneProteinPurificationKit
17-5468-15	HiScreen Capto SP ImpRes
17-5470-15	HiScreen Capto Q ImpRes
17-5474-15	HiScreen MabSelect SuRe LX

Glutathione Sepharose 4 Fast Flow - Scale-up Purification

• Fast, one-step purification of GST*-tagged proteins and other glutathione S-transferase or glutathione-dependent proteins.
• Glutathione coupled to Sepharose™ 4 Fast Flow via a 10-carbon spacer arm.
• Excellent for scale-up due to high capacity and high flow properties.

Technical Information

Glutathone Sepharose 6 Fast Flow for purification of GST-tagged proteins.

 

Glutathione Sepharose™ 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose™ 4 Fast Flow provides good flow properties and is excellent for scaling up.

For more information of the full range of products included in the Glutathione S-transferase (GST*) Gene Fusion System, see Introduction to Glutathione S-Transferase (GST) Gene Fusion System. A more comprehensive guide to the GST Gene Fusion System is available in handbook 18-1157-58, see Media Reference Books, Software and Video Cassettes.

 

TECHNICAL SPECIFICATIONS
Ligand	Glutathione
Ligand coupling method	Epoxy activation
Matrix	Highly cross-linked agarose, 4%
Average particle size	90 μm
Binding capacity*	» 10 mg recombinant GST/ml medium
pH stability	3-12
Chemical stability	All commonly used aqueous buffers, e.g. 1 M acetate, pH 6.0, 0.1 M NaOH,  70% ethanol, 6 M guanidine hydrochloride (1 h at room temperature)
Storage	20% ethanol
Storage temperature	4°C to 30°C
*Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.