17-1273-01	SOURCE 30S, 50 ML
17-1275-01	SOURCE 30Q, 50 ML
17-1287-01	ANX SEPH 4 FF HIGH SUB 500ML
17-1287-10	ANX SEPHAROSE FF HIGHSUB 25 ML
17-5072-01	Q SEPHAROSE XL, 300 ML
17-5073-01	SP SEPHAROSE XL, 300 ML
17-5316-02	Capto Q 100 ml
17-5316-10	CAPTO Q, 25 ML
17-5317-02	Capto MMC 100ml
17-5317-10	CAPTO MMC, 25ML
17-5432-01	Butyl Sepharose HP, 25 ml
17-5432-02	Butyl Sepharose HP, 200ml
17-5437-01	Q SEPHAROSE XL VIRUS LIC 300ML
17-5437-10	Q SEPHAROSE XL VIRUS LIC 25 ML
17-5441-01	CAPTO S, 100 ML
17-5441-10	CAPTO S, 25 ML
17-5443-01	CAPTO DEAE, 100 ML
17-5443-10	CAPTO DEAE, 25 ML
17-5444-10	CAPTO adhere 25 ML

SOURCE™ Media/RESOURCE™ and Tricorn Columns for IEX

• SOURCE 15Q and 15S media are ideal for fast, high-resolution purifications and easy scale-up.
• Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/ml, and improved stability in organic solvents and pH extremes.
• SOURCE 15Q 4.6/100 PE and SOURCE 15S 4.6/100 PE are Tricorn high-performance columns designed for high-resolution purification at lab scale and for optimization studies when scaling up.
• RESOURCE columns are prepacked with SOURCE 15Q or SOURCE 15S for fast separation. Separation times using 1 ml RESOURCE columns are less than 3 min at 9.6 ml/min and about 20 min using a peristaltic pump at 1 ml/min.
• SOURCE 30Q and 30S media are designed for intermediate purification and large-scale polishing.

SOURCE™ Media/RESOURCE™ and Tricorn Columns for IEX

Technical Information

SOURCE™ Media, RESOURCE™, and Tricorn prepacked ion exchange columns increase productivity.

 

TECHNICAL SPECIFICATIONS
SOURCE media
Ion exchanger type
SOURCE™ 15Q, 30Q	Quaternary ammonium strong anion
SOURCE™ 15S, 30S	Methyl sulfonate strong cation
Particle size
SOURCE™ 15Q, 15S	15 μm
SOURCE™ 30Q, 30S	30 μm
Matrix	Polystyrene/divinylbenzene
pH stability	2-12 (long term), 1-14 (short term)
Chemical stability	Stable in all common ion exchange buffers
Storage	20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (S)
Storage temperature	4°C to 30°C
RESOURCE™ prepacked columns
Bed volume	1 ml or 6 ml
Bed diameter	6.4 mm (1-ml column) 16 mm (6-ml column)
Bed height	30 mm
Max. pressure	1-ml column: 15 bar (218 psi, 1.5 MPa) 6-ml column: 6 bar (87 psi, 0.6 MPa)
Max. flow rate*	1800 cm/h (10 ml/min for 1-ml column) 1800 cm/h (60 ml/min for 6-ml column)
SOURCE 4.6/100 PE columns (Tricorn)†
Bed volume	1.7 ml
Bed dimensions	4.6 mm × 100 mm
Max. pressure	40 bar (580 psi, 4 MPa)
Max. flow rate*	5 ml/min (1800 cm/h)
* H2O at 25°C † Columns not suitable for use with ÄKTAprime™ plus system.

 

Four different pH-values were tested for high resolution separation of recombinant protein DnaK.

 

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