英文名称： HITRAP IEX SELECTION KIT
| 17-6002-33 | HITRAP IEX SELECTION KIT |
| 18-1142-75 | REC PROTEIN PURIF HANDBOOK |
| 28-4010-81 | HITRAP_HIPREP 1_16 CONNEC AKTA |
| 28-4014-77 | HISTRAP FF CRUDE KIT |
| 28-4017-45 | GSTRAP 4B, 5 X 1 ML |
| 28-4017-46 | GSTRAP 4B, 100 X 1 ML |
| 28-4017-47 | GSTRAP 4B, 1 X 5 ML |
| 28-4017-48 | GSTRAP 4B, 5 X 5 ML |
| 28-4017-49 | GSTRAP 4B, 100 X 5 ML |
| 28-4058-44 | HiTrap Capto Adhere 5x1ml |
| 28-4058-46 | 5x5 ml Hitrap Capto adhere |
| 28-4082-53 | HITRAP MABSELECT, 5 X 1 ML |
| 28-4082-55 | HITRAP MABSELECT, 1 X 5 ML |
| 28-4082-56 | HITRAP MABSELECT, 5 X 5 ML |
| 28-4082-58 | HITRAP MABSELECT XTRA, 5X1ML |
| 28-4082-60 | HITRAP MABSELECT XTRA, 1X5ML |
| 28-4082-61 | HITRAP MABSELECT XTRA, 5X5ML |
| 28-4110-01 | HiTrap Butyl HP, 5x1ml |
| 28-4110-05 | HiTrap Butyl HP, 5 x 5ml |
| 28-4110-07 | HiTrap HIC Selection Kit, 7x1 |
| 28-9075-46 | StrepTrap HP 5 x 1mL |
| 28-9075-47 | StrepTrap HP 1 x 5mL |
| 28-9075-48 | StrepTrap HP 5 x 5mL |
| 28-9165-37 | HiTrap Capto DEAE, 5X1ML |
| 28-9165-40 | HiTrap Capto DEAE, 5X5ML |
| 28-9187-78 | MBPTrap 5 x 1mL |
| 28-9187-79 | MBPTrap 1 x 5 mL |
| 28-9187-80 | MBPTrap 5 x 5mL |
| 28-9343-88 | HiTrap Capto IEX Selection Kit |
| 28-9520-85 | HiTrap Con A 4B 5 x 1 ml |
| 28-9520-96 | HiTrap Con A 4B 5 x 5 ml |
HiTrap™ IEX Selection Kit
- Fast and easy selection with seven different ion-exchange ligands on Sepharose™ Fast Flow and Sepharose™ XL, prepacked in 1-ml HiTrap™ columns.
- Excellent for optimization studies: buffer composition, pH, flow rate, sample loading, and elution scheme can be optimized with small sample quantities prior to scale-up.
- Excellent for screening different media: includes seven 1-ml HiTrap™ columns prepacked with Q Sepharose™ Fast Flow (strong anion), SP Sepharose™ Fast Flow (strong cation), CM Sepharose™ Fast Flow (weak cation), DEAE Sepharose™ Fast Flow (weak anion), ANX Sepharose™ 4 Fast Flow (high sub) (weak anion), Q Sepharose™ XL (strong anion), and SP Sepharose™ XL (strong cation), connectors, and instructions.
- Designed for use with syringe, peristaltic pump and other chromatography systems such as ÄKTA™design.
HiTrap™ IEX Selection Kit
Technical Information
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Q, SP, DEAE, and CM Sepharose™ Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities.
ANX Sepharose™ 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.
Q and SP Sepharose™ XL media have long chains of *** coupled to a robust, 6% highly cross-linked agarose matrix. The *** chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities.
The active end of the charged group is the same for DEAE Sepharose™ Fast Flow and ANX Sepharose™ Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose™ Fast Flow has a diethylaminoethyl-group bound to the agarose whilst ANX Sepharose™ 4 Fast Flow (high sub) has a diethylaminopropyl group attached.
| TECHNICAL SPECIFICATIONS | |
| Media | Q Sepharose™ Fast Flow |
| SP Sepharose™ Fast Flow | |
| CM Sepharose™ Fast Flow | |
| DEAE Sepharose™ Fast Flow | |
| ANX Sepharose™ 4 Fast Flow (high sub) | |
| Q Sepharose™ XL | |
| SP Sepharose™ XL | |
| Column volume | 1 ml |
| Max. flow rate* | 4 ml/min |
| Recommended flow rate | 1 ml/min |
| Max. back pressure | 3 bar (0.3 MPa, 43 psi) |
| Storage | 20% ethanol (Q, DEAE, ANX, CM), 0.2 M sodium acetate in 20% ethanol (SP) |
| Storage temperature | 4°C to 30°C |
| * H2O at 25°C
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