HiPrep 16/60 Sephacryl S-400 H
英文名称： HiPrep 16/60 Sephacryl S-400 H
型号:： 28-9356-04
价格:请致电：010-57128832,18610462672
品牌: 瑞典

28-9356-04 HiPrep 16/60 Sephacryl S-400 H
28-9356-05 HiPrep 26/60 Sephacryl S-400 H
28-9356-06 HiPrep 16/60 Sephacryl S-500 H
28-9356-07 HiPrep 26/60 Sephacryl S-500 H
28-9365-38 HiPrep Q XL 16/10
28-9365-40 HiPrep SP XL 16/10
28-9365-41 HiPrep DEAE FF 16/10
28-9365-42 HiPrep CM FF 16/10
28-9365-43 HiPrep Q FF 16/10
28-9365-44 HiPrep SP FF 16/10
28-9365-45 HiPrep Phenyl FF (high sub) 16
28-9365-46 HiPrep Phenyl FF (low sub) 16/
28-9365-47 HiPrep Butyl FF 16/10
28-9365-48 HiPrep Octyl FF 16/10
28-9365-49 HiPrep Heparin FF 16/10
28-9365-50 GSTPrep FF 16/10
28-9365-51 HisPrep FF 16/10
28-9365-52 HiPrep IMAC FF 16/10

HiPrep™ Sephacryl™ HR Columns and Sephacryl™ Lab Packs

  • Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl ***/ bisacrylamide matrix.
  • Sephacryl™ S-100 HR is designed for separating peptides and small proteins.
  • S-200 HR and S-300 HR are for purifying antibodies, serum proteins, and mid-size proteins.
  • S-400 HR and S-500 HR are used to separate polysaccharides, macromolecules with extended structures, and even small plasmids.
  • S-1000 SF is optimized for the purification of ***s up to 108 molecular weight, spherical particles up to 400 nm, and DNA up to 20 000 base pairs including plasmids, vesicles, and viruses.
  • Convenient, prepacked HiPrep™ Sephacryl™ columns speed up protein purification.

  • HiPrep™ Sephacryl™ HR Columns and Sephacryl™ Lab Packs

    Technical Information


    Click here to view this image in high resolution
    HiPrep™ Sephacryl™ HR columns, prepacked with Sephacryl™ High Resolution media, provide reliable gel filtration separations.

     

    HiPrep™ Sephacryl™ HR prepacked gel filtration columns offer excellent separation properties in a convenient, ready-to-use format. By combining the performance of Sephacryl™ High Resolution media with a low cost column construction, they are both reliable and cost effective to use with simple equipment or systems.

     

    Five HiPrep™ Sephacryl™ HR columns are available prepacked with S-100, S-200, S-300, S-400, or S-500 HR media, respectively. Each column is also available in two sizes: 16/60 for sample volumes of up to 5 ml and 26/60 for sample volumes of up to 13 ml. The range of HiPrep™ Sephacryl™ HR prepacked columns allows purification of molecules with molecular weights as small as 1 × 103 to macromolecules as large as 2 × 107.

     

    TECHNICAL SPECIFICATIONS
    Sephacryl™ High Resolution
    Fractionation range (Mr) -   
    - Globular proteins   
      S-100 HR
      S-200 HR
      S-300 HR
      S-400 HR      		 1 × 103 - 1 × 105
    5 × 103 - 2.5 × 105
    1 × 104 - 1.5 × 106
    2 × 104 - 8 × 106 
    - Dextrans   
      S-200 HR
      S-300 HR
      S-400 HR
      S-500 HR
      S-1000 HR      		 1 × 103 - 8 × 104
    2 × 103 - 4 × 105
    1 × 104 - 2 × 106
    4 × 104 - 2 × 107
    5 × 105 - > 108 
      Exclusion limit up to 400 nm for spherical particles. 
    - DNA exclusion limit   
      S-200 HR
      S-300 HP
      S-400 HR
      S-500 HR
      S-1000 HR     		 30 bp
    118 bp
    271 bp
    1078 bp
    20 000 bp 
    Matrix  Spherical allyl *** and N, N’-methylenebisacrylamide 
    Chemical stability  Stable in all common buffers:
    1 M acetic acid, 8 M urea,
    6 M guanidine-HCl, 1% SDS,
    2 M NaCl, 24% ethanol,
    30% propanol, 30% acetonitrile,
    0.5 M NaOH (for cleaning) 
    pH stability  3-11 (long term and working)
    2-13 (short term) 
    Storage  20% ethanol 
    Storage temperature  4°C to 30°C 
    Physical stability
      Autoclavability
      Antimicrobial agent     		 Negligible volume variation due to changes in pH or ionic strength
      At 121°C, pH 7 for 30 minutes (gel only)
      20% ethanol 
      Autoclavability    At 121°C, pH 7 for 30 minutes (gel only) 
      Autoclavability    At 121°C, pH 7 for 30 minutes (gel only) 
      Antimicrobial agent    20% ethanol 
      Autoclavability    At 121°C, pH 7 for 30 minutes (gel only) 
      Antimicrobial agent    20% ethanol 
      Autoclavability    At 121°C, pH 7 for 30 minutes (gel only) 
      Antimicrobial agent    20% ethanol 

     

    Click here to view this image in high resolution
    Selectivity of the five different prepacked HiPrep Sephacryl™ HR columns.

     

    TECHNICAL SPECIFICATIONS
    Prepacked HiPrep™ Sephacryl HR Columns
    Bed dimensions   
      HiPrep™ 16/60
      HiPrep™ 26/60     		 16 × 600 mm
    26 × 600 mm 
    Bed volume   
      HiPrep™ 16/60
      HiPrep™ 26/60     		 120 ml
    320 ml 
    Sample volume   
      HiPrep™ 16/60
      HiPrep™ 26/60     		 < 5 ml
    < 13 ml     
    Rec. linear flow rate  5-15 cm/h 
    Max. linear flow rate  30 cm/h 
    (H2O at 25°C)   
    Max. pressure over the packed bed during operation  1.5 bar (22 psi, 0.15 MPa) 
    Column hardware pressure limit  5 bar (73 psi, 0.5 MPa) 
    Theoretical plates  5000 m-1 
    Storage  20% ethanol 
    Storage temperature  4°C to 30°C