17-5112-01	HITRAP STREPTAVIDIN HP 5X1 ML
17-5130-01	GSTRAP FF, 5X1ML
17-5130-02	GSTRAP FF, 2X1ML
17-5130-05	GSTRAP FF, 100 X 1 ML
17-5131-01	GSTRAP FF, 1X5ML
17-5131-02	GSTRAP FF 5 X 5 ML
17-5131-05	GSTRAP FF, 100 X 5 ML
17-5143-01	HITRAP BENZAMIDINE FF 5 X 1ML
17-5143-02	HITRAP BENZAMIDINE FF 2 X 1 ML
17-5144-01	HITRAP BENZAMIDINE FF 1 X 5 ML
17-5154-01	HITRAP DEAE FF 5 X 5 ML
17-5155-01	HITRAP CM FF, 5 X 5 ML
17-5156-01	HITRAP Q FF, 5 X 5 ML
17-5157-01	HITRAP SP FF, 5 X 5 ML
17-5158-01	HITRAP Q XL, 5 X 1 ML
17-5159-01	HITRAP Q XL, 5 X 5 ML
17-5160-01	HITRAP SP XL, 5 X 1 ML
17-5161-01	HITRAP SP XL, 5 X 5 ML
17-5162-01	HITRAP ANX FF (HIGHSUB)5 X 1ML
17-5163-01	HITRAP ANX FF(HIGHSUB) 5 X 5ML
17-5193-01	HITRAP PHENYL FF (HS), 5 X 5ML
17-5194-01	HITRAP PHENYL FF (LS), 5X 5ML
17-5195-01	HITRAP PHENYL HP, 5 X 5 ML
17-5196-01	HITRAP OCTYL FF, 5 X 5 ML
17-5197-01	HITRAP BUTYL FF, 5 X 5 ML
17-5247-01	HISTRAP HP 5 X 1 ML
17-5247-05	HISTRAP HP 100 X 1 ML
17-5248-01	HISTRAP HP 1 X 5 ML
17-5248-02	HISTRAP HP 5 X 5 ML
17-5248-05	HISTRAP HP 100 X 5 ML
17-5255-01	HISTRAP FF 5 X 5 ML
17-5255-02	HISTRAP FF 100 X 5 ML
17-5281-01	GSTRAP HP 5 X 1 ML
17-5281-05	GSTRAP HP 100 X 1 ML
17-5282-01	GSTRAP HP 1 X 5 ML
17-5282-02	GSTRAP HP 5 X 5 ML
17-5282-05	GSTRAP HP, 100 X 5 ML
17-5286-01	HISTRAP FF CRUDE 5X5 ML
17-5286-02	HISTRAP FF CRUDE 100X5 ML
17-5319-01	HISTRAP FF 5 X 1 ML
17-5319-02	HISTRAP FF 100 X 1 ML
17-5441-22	HITRAP CAPTO S, 5X1 ML
17-5441-23	HITRAP CAPTO S, 5X5 ML
17-5468-51	HiTrap Capto SP ImpRes 5x1mL
17-5468-55	HiTrap Capto SP ImpRes 5x5mL
17-5470-51	HiTrap Capto Q ImpRes 5x1mL
17-5470-55	HiTrap Capto Q ImpRes 5x5mL
17-6002-33	HITRAP IEX SELECTION KIT
18-1142-75	REC PROTEIN PURIF HANDBOOK
28-4010-81	HITRAP_HIPREP 1_16 CONNEC AKTA
28-4014-77	HISTRAP FF CRUDE KIT
28-4017-45	GSTRAP 4B, 5 X 1 ML
28-4017-46	GSTRAP 4B, 100 X 1 ML
28-4017-47	GSTRAP 4B, 1 X 5 ML
28-4017-48	GSTRAP 4B, 5 X 5 ML
28-4017-49	GSTRAP 4B, 100 X 5 ML
28-4058-44	HiTrap Capto Adhere 5x1ml
28-4058-46	5x5 ml Hitrap Capto adhere
28-4082-53	HITRAP MABSELECT, 5 X 1 ML
28-4082-55	HITRAP MABSELECT, 1 X 5 ML
28-4082-56	HITRAP MABSELECT, 5 X 5 ML
28-4082-58	HITRAP MABSELECT XTRA, 5X1ML
28-4082-60	HITRAP MABSELECT XTRA, 1X5ML
28-4082-61	HITRAP MABSELECT XTRA, 5X5ML
28-4110-01	HiTrap Butyl HP, 5x1ml
28-4110-05	HiTrap Butyl HP, 5 x 5ml
28-4110-07	HiTrap HIC Selection Kit, 7x1
28-9075-46	StrepTrap HP 5 x 1mL
28-9075-47	StrepTrap HP 1 x 5mL
28-9075-48	StrepTrap HP 5 x 5mL
28-9165-37	HiTrap Capto DEAE, 5X1ML
28-9165-40	HiTrap Capto DEAE, 5X5ML
28-9187-78	MBPTrap 5 x 1mL
28-9187-79	MBPTrap 1 x 5 mL
28-9187-80	MBPTrap 5 x 5mL
28-9343-88	HiTrap Capto IEX Selection Kit
28-9520-85	HiTrap Con A 4B 5 x 1 ml
28-9520-96	HiTrap Con A 4B 5 x 5 ml

HiTrap Streptavidin HP Columns

• Convenient, fast, reliable purification of biotinylated compounds

HiTrap Streptavidin HP Columns

Technical Information

HiTrap™ Streptavidin HP columns are prepacked with Streptavidin Sepharose™ High Performance, which exploits the strong interactions of biotin and streptavidin, as well as the somewhat weaker interactions of 2-iminobiotin and streptavidin. The prepacked Streptavidin Sepharose™ High Performance medium ensures reproducibility, high performance, and excellent physical and chemical stability. Streptavidin Sepharose™ High Performance is also available in lab packs, using the same purified streptavidin isolated from Streptomyces avidinii bound to Sepharose™ High Performance.

 

Complete instructions for use and method optimization are included, as are a variety of connectors for syringe, peristaltic pump, and chromatography systems such as ÄKTA™design.

 

HiTrap™ Streptavidin HP Columns

 

TECHNICAL SPECIFICATIONS
Ligand	Streptavidin
Matrix	Highly cross-linked agarose, 6%
Average particle size	34 μm
Binding capacity	> 300 nmol biotin/ml medium 6 mg biotinylated BSA/ml medium
pH stability	4-9 (long term), 2-10.5 (short term)
Storage	20% ethanol, 0.1 M potassium phosphate buffer, pH 8.0
Storage temperature	4°C to 30°C

 

Sample:	9.0 ml of a mixture of BSA and iminobiotinylated BSA. Filtered through a 0.45 μm filter.
Column:	HiTrap™ Streptavidin HP 1 ml
Binding buffer:	50 mM ammonium carbonate, 0.5 M NaCl, pH 10.0
Elution buffer:	50 mM ammonium acetate, 0.5 M NaCl, pH 4.0
Flow rate:	1 ml/min (0.3 ml/min during sample application)
Instrumentation:	ÄKTAexplorer™ 10 S

 

Purification of iminobiotinylated BSA on HiTrap™ Streptavidin HP 1 ml.

http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=12733