HisPrep FF 16/10 columns are prepacked with Ni Sepharose 6 Fast Flow for scale-up purification of histidine-tagged proteins. Ni Sepharose 6 Fast Flow consists of 90-μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni²⁺ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni²⁺ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

HisPrep™ FF 16/10 column for convenient scale-up purification of histidine-tagged proteins.

Customer feedback on Ni Sepharose 6 Fast Flow.

* For licensing information, see Legal Info.

HiPrep IMAC FF 16/10, see IMAC Sepharose 6 Fast Flow Media/HiTrap IMAC FF Columns/HiPrep IMAC FF 16/10 Column/HiScreen IMAC FF Column, is the product of choice when charging the medium with different metal ions for optimization of purification protocols.

For the complete range of products for purification of histidine-tagged proteins, see Introduction to Tagged Protein Purification.

HisPrep™ FF 16/10 Column - Easy Scale-up Purification

Technical Information

TECHNICAL SPECIFICATIONS
Medium	Ni Sepharose 6 Fast Flow
Column volume	20 ml
Dynamic binding capacity*	Approx. 40 mg histidine-tagged protein/ml medium
Column dimensions	1.6 × 10 cm
Recommended flow rate†	2-10 ml/min (60-300 cm/h)
Max. flow rate†	10 ml/min (300 cm/h)
Max. pressure over the packed bed during operation, Dp	0.15 MPa, 1.5 bar
Column hardware pressure limit	0.5 MPa, 5 bar
Compatibility	Stable in all commonly used buffers, reducing agents, denaturants, and detergents, see Introduction to Tagged Protein Purification for more information.
Storage	20% ethanol
Storage temperature	4°C to 30°C
* Binding capacity is protein-to-protein dependent † H2O at room temperature

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