英文名称： HiTrap Capto Adhere
| 28-4058-44 | HiTrap Capto Adhere 5x1ml |
| 28-4058-46 | 5x5 ml Hitrap Capto adhere |
| 28-4082-53 | HITRAP MABSELECT, 5 X 1 ML |
| 28-4082-55 | HITRAP MABSELECT, 1 X 5 ML |
| 28-4082-56 | HITRAP MABSELECT, 5 X 5 ML |
| 28-4082-58 | HITRAP MABSELECT XTRA, 5X1ML |
| 28-4082-60 | HITRAP MABSELECT XTRA, 1X5ML |
| 28-4082-61 | HITRAP MABSELECT XTRA, 5X5ML |
| 28-4110-01 | HiTrap Butyl HP, 5x1ml |
| 28-4110-05 | HiTrap Butyl HP, 5 x 5ml |
| 28-4110-07 | HiTrap HIC Selection Kit, 7x1 |
| 28-9075-46 | StrepTrap HP 5 x 1mL |
| 28-9075-47 | StrepTrap HP 1 x 5mL |
| 28-9075-48 | StrepTrap HP 5 x 5mL |
| 28-9165-37 | HiTrap Capto DEAE, 5X1ML |
| 28-9165-40 | HiTrap Capto DEAE, 5X5ML |
| 28-9187-78 | MBPTrap 5 x 1mL |
| 28-9187-79 | MBPTrap 1 x 5 mL |
| 28-9187-80 | MBPTrap 5 x 5mL |
| 28-9343-88 | HiTrap Capto IEX Selection Kit |
| 28-9520-85 | HiTrap Con A 4B 5 x 1 ml |
| 28-9520-96 | HiTrap Con A 4B 5 x 5 ml |
Capto adhere/HiTrap Capto adhere
- Contaminant removal to formulation levels in post protein A purification
- Prepacked HiTrap columns for easy screening and convenient process development.
- High capacity and productivity in flow-through mode
- Wide operational window of pH and conductivity
- Savings in time and operating costs with a two-step chromatographic process
Capto adhere/HiTrap Capto adhere
Technical Information
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Capto adhere is a strong anion exchanger with multimodal functionality. The multimodal functionality gives a different selectivity compared to traditional anion exchangers. Capto adhere is designed for post Protein A purification of monoclonal antibodies. Removal of leached Protein A, aggregates, host cell proteins, nucleic acids and viruses from monoclonal antibodies is performed in flow-through mode at which the antibodies pass directly through the column while the contaminants are adsorbed. For best performance of Capto adhere, screening/optimization of loading conditions are required.
| TECHNICAL SPECIFICATIONS - Capto™ adhere | |
| Ion exchanger type | Multimodal strong anion exchanger |
| Ionic capacity | 0.09 to 0.12 mmol Cl-/ml medium |
| Average particle size1 | 75 μm (d50V) |
| Matrix | Highly cross-linked agarose |
| Flow velocity2 | At least 600 cm/h in a 1-m diameter column with 20-cm bed height at 20°C using process buffers with the same viscosity as water at < 3 bar (0.3 MPa) |
| pH-stability3 | 2 to 14 (short term) 3 to 12 (long term) |
| Working temperature4 | 4°C to 30°C |
| Chemical stability5 | All commonly used aqueous buffers, 1 M acetic acid, 1 M sodium hydroxide |
| Avoid | Oxidizing agents, anionic detergents |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
| 1 d50v is the median particle size of the cumulative volume distribution. 2 The capacity for selective removal of some key contaminants may decrease at high flow velocity. 3 Short term pH: pH interval where the medium can be subjected to, for cleaning- or sanitization-in-place. Long term pH: pH interval where the medium can be operated without significant change in function. 4 Capto adhere can be used under cold-room conditions, but the capacity for some key contaminants may decrease. 5 No significant change in ionic binding capacity and carbon content after one week storage in 1 M NaOH at 40°C.
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