17-0406-01	HITRAP HEPARIN HP, 5 X 1 ML
17-0407-01	HITRAP HEPARIN HP,1 X 5 ML
17-0407-03	HiTrap Heparin HP 5x5ml
17-0408-01	HITRAP CHELATING HP, 5 X 1 ML
17-0409-01	HITRAP CHELATING HP, 1 X 5 ML
17-0409-03	HITRAP CHELATING HP 5 X 5 ML
17-0409-05	HITRAP CHELATING HP 100 X 5 ML
17-0412-01	HITRAP BLUE HP, 5 X 1 ML
17-0413-01	HITRAP BLUE HP, 1 X 5 ML
17-0716-01	HITRAP NHS-ACTIV HP, 5 X 1ML
17-0716-30	HiTrap NHS-activated HP, 180 x
17-0717-01	HITRAP NHS-ACTIV HP, 1 X 5 ML
17-0920-03	HITRAP IMAC HP, 5 X 1 ML
17-0920-05	HITRAP IMAC HP, 5 X 5 ML
17-0921-02	HITRAP IMAC FF, 5 X 1ML
17-0921-04	HITRAP IMAC FF, 5 X 5 ML
17-0978-13	HITRAP BUTYL-S FF, 5 X 1ML
17-0978-14	HITRAP BUTYL-S FF, 5 X 5ML

HiTrap™ Heparin HP Columns

• Prepacked with Heparin Sepharose™ High Performance for purification of proteins such as DNA-binding proteins, coagulation factors, lipoproteins, and protein synthesis factors.
• Stable over pH 5-10 and tolerates all commonly used aqueous buffers.

HiTrap™ Heparin HP Columns

Technical Information

Immobilized heparin has two main modes of interaction with proteins. It can operate as an affinity ligand; e.g. in its interaction with coagulation factors. Heparin also functions as a high capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases. Heparin is coupled to the Sepharose™ High Performance base matrix via the N-hydroxysuccinamide coupling method, to give high capacity, performance, and low leakage levels. HiTrap™ Heparin is delivered in 0.2 M NaCl with 20% ethanol as bacteriostatic agent. Store HiTrap™ Heparin columns equilibrated with 25 ml 20% ethanol or a buffer containing a bacteriostatic agent. Equilibration is easily done with a syringe and the supplied luer adapter. Seal the column with the supplied stopper and domed nut. The recommended storage temperature is 4 to 30°C.

 

TECHNICAL SPECIFICATIONS
Ligand	Porcine heparin
Ligand coupling method	Reductive amination
Ligand density	» 10 mg heparin/ml medium
Matrix	Highly cross-linked agarose, 6%
Average particle size	34 μm
pH stability	pH 5-10
Storage	20% ethanol
Storage temperature	4°C to 30°C

 

Purification of Antithrombin III from bovine plasma.

 

SDS -PAGE electrophoresis with PhastSystem™ and PhasGel Gradient 8-25, silver stained