英文名称： HITRAP HEPARIN HP
| 17-0406-01 | HITRAP HEPARIN HP, 5 X 1 ML |
| 17-0407-01 | HITRAP HEPARIN HP,1 X 5 ML |
| 17-0407-03 | HiTrap Heparin HP 5x5ml |
| 17-0408-01 | HITRAP CHELATING HP, 5 X 1 ML |
| 17-0409-01 | HITRAP CHELATING HP, 1 X 5 ML |
| 17-0409-03 | HITRAP CHELATING HP 5 X 5 ML |
| 17-0409-05 | HITRAP CHELATING HP 100 X 5 ML |
| 17-0412-01 | HITRAP BLUE HP, 5 X 1 ML |
| 17-0413-01 | HITRAP BLUE HP, 1 X 5 ML |
| 17-0716-01 | HITRAP NHS-ACTIV HP, 5 X 1ML |
| 17-0716-30 | HiTrap NHS-activated HP, 180 x |
| 17-0717-01 | HITRAP NHS-ACTIV HP, 1 X 5 ML |
| 17-0920-03 | HITRAP IMAC HP, 5 X 1 ML |
| 17-0920-05 | HITRAP IMAC HP, 5 X 5 ML |
| 17-0921-02 | HITRAP IMAC FF, 5 X 1ML |
| 17-0921-04 | HITRAP IMAC FF, 5 X 5 ML |
| 17-0978-13 | HITRAP BUTYL-S FF, 5 X 1ML |
| 17-0978-14 | HITRAP BUTYL-S FF, 5 X 5ML |
HiTrap™ Heparin HP Columns
- Prepacked with Heparin Sepharose™ High Performance for purification of proteins such as DNA-binding proteins, coagulation factors, lipoproteins, and protein synthesis factors.
- Stable over pH 5-10 and tolerates all commonly used aqueous buffers.
HiTrap™ Heparin HP Columns
Technical Information
)
Immobilized heparin has two main modes of interaction with proteins. It can operate as an affinity ligand; e.g. in its interaction with coagulation factors. Heparin also functions as a high capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases. Heparin is coupled to the Sepharose™ High Performance base matrix via the N-hydroxysuccinamide coupling method, to give high capacity, performance, and low leakage levels. HiTrap™ Heparin is delivered in 0.2 M NaCl with 20% ethanol as bacteriostatic agent. Store HiTrap™ Heparin columns equilibrated with 25 ml 20% ethanol or a buffer containing a bacteriostatic agent. Equilibration is easily done with a syringe and the supplied luer adapter. Seal the column with the supplied stopper and domed nut. The recommended storage temperature is 4 to 30°C.
| TECHNICAL SPECIFICATIONS | |
| Ligand | Porcine heparin |
| Ligand coupling method | Reductive amination |
| Ligand density | » 10 mg heparin/ml medium |
| Matrix | Highly cross-linked agarose, 6% |
| Average particle size | 34 μm |
| pH stability | pH 5-10 |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
)
Purification of Antithrombin III from bovine plasma.
)
SDS -PAGE electrophoresis with PhastSystem™ and PhasGel Gradient 8-25, silver stained