17-1064-01	HILOAD 16/10 Q SEPHAROSE HP
17-1066-01	HILOAD 26/10 Q SEPHAROSE HP
17-1085-01	HILOAD 16/10 PHENYL SEPHAROSE
17-1086-01	HILOAD 26/10 PHENYL SEPHAROSE

 

 
 

TECHNICAL SPECIFICATIONS
Ion exchanger type
Q Sepharose™ HP	Quaternary ammonium strong anion exchanger
SP Sepharose™ HP	Sulfopropyl strong cation exchanger
Charged groups
SP Sepharose™ HP	- SO3-
Q Sepharose™ HP	- N+(CH3)3
Ionic capacity
Q Sepharose™ HP	0.14-0.20 mmol Cl-/ml
SP Sepharose™ HP	0.15-0.20 mmol H+/ml
Dynamic capacity
Q Sepharose™ HP	70 mg BSA/ml medium
SP Sepharose™ HP	55 mg ribonuclease/ml medium
Rec. flow velocity	30-150 cm/h
Max. pressure over the packed bed in a HiLoad column during operation	3 bar (42 psi, 0.3 MPa)
HiLoad™ column hardware pressure limit	5 bar (73 psi, 0.5 MPa)
Average particle size	34 μm
Exclusion limit (Mr)	» 4 ×106 globular protein
Matrix	cross-linked agarose, 6%
pH stability
Q Sepharose™ HP	2-12 (working and long term), 1-14 (short term)
SP Sepharose™ HP	4-13 (working and long term), 3-14 (short term)
Chemical stability	Stable in all commonly used buffers:  8 M urea, 6 M guanidine HCl, 70% ethanol, 1 M NaOH,* 1 M acetic acid*
Storage	20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (SP)
Storage temperature	4°C to 30°C
Prepacked HiLoad™ Columns
Bed volume
HiLoad™ 16/10	20 ml
HiLoad™ 26/10	53 ml
Bed dimensions
HiLoad™ 16/10	16 mm × 100 mm
HiLoad™ 26/10	26 mm × 100 mm
Efficiency	> 12 000 m-1
* 1 M NaOH and 1 M acetic acid should only be used for cleaning.

 

Separation of acyl-CoA-binding protein on Q Sepharose™ High Performance

http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=9041