英文名称： MONO Q PC 1.6/5
| 17-0671-01 | MONO Q PC 1.6/5 |
| 17-0672-01 | MONO S PC 1.6/5 |
| 17-0673-01 | SUPEROSE 6 PC 3.2/30 |
| 17-0674-01 | SUPEROSE 12 PC 3.2/30 |
| 17-0686-01 | MINI Q PC 3.2/3 |
| 17-0687-01 | MINI S PC 3.2/3 |
| 17-0771-01 | SUPERDEX 75 PC 3.2/30 |
| 17-1089-01 | SUPERDEX 200 PC 3.2/30 |
| 17-1455-01 | PRECISION COLUMN HOLDER |
| 17-1458-01 | SUPERDEX PEPTIDE PC 3.2/30 |
| 17-1550-01 | COLUMN HOLDER, 10 CM |
| 18-1015-28 | PROTECTIVE CAPS (2/PK) |
| 17-5057-01 | MICRORPC C2-C18 ST 4,6-100 |
| 17-5068-01 | SOURCE 15RPC ST 4.6/100 |
| 17-5116-01 | SOURCE 5RPC ST 4,6-150 |
| 17-5172-01 | SUPEROSE 6 10/300 GL |
| 17-5173-01 | SUPEROSE 12 10/300 GL |
| 17-5174-01 | SUPERDEX 75 10/300 GL |
| 17-5175-01 | SUPERDEX 200 10/300 GL |
| 17-5176-01 | SUPERDEX PEPTIDE 10/300 GL |
| 28-9065-61 | Superdex 200 5_150 GL |
| 28-9205-04 | Superdex 75 5_150 GL |
MonoBeads™ Columns
- Innovative, monodisperse 10-μm porous beads yield high resolution, dynamic capacity, reproducibility, and durability.
- MonoBeads™ available in a choice of three prepacked column formats¾Tricorn™, HR, and PC columns.
- Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q and S ion exchangers.
- Compatible with ÄKTAdesign™, FPLC™ System, and other high-performance LC systems.
- MonoBeads™ are also available as Mono P for chromatofocusing (see Mono P™ Columns).
MonoBeads™ Columns
Technical Information
MonoBeads™ are prepacked in Tricorn™, HR, and PC column formats. The three Tricorn™ (5/50 GL, 10/100 GL, and 4.6/100 PE) columns offer high-resolution separations with greater loading capacity but slightly lower resolution than MiniBeads™ columns (see page 511).
Tricorn™ columns are ideal for the polishing step when high purity is required. HR 16/10 columns, on the other hand, have higher loading capacity than Tricorn™ columns. PC 1.6/5 columns are ideal for micropurification of subnanogram to microgram amounts of sample on ÄKTA Systems or other high-performance LC systems.
Customer feedback on Mono Q HR 5/5.
| TECHNICAL SPECIFICATIONS | ||
| MonoBeads™ | ||
| Ion exchanger type | Mono Q™ | Strong anion |
| Mono S™ | Strong cation | |
| Ionic capacity | Mono Q™ | 0.27-0.37 mmol Cl-/ml |
| Mono S™ | 0.12-0.15 mmol H+/ml | |
| pH stability | 2-12 (working), 1-14 (short term) | |
| Chemical stability | Stable in all common buffers except high concentration oxidizing and reactive agents, DMSO, DMF, and formic acid. | |
| Medium | Monodisperse polystyrene/divinyl benzene particles | |
| Particle size | 10 μm | |
| Storage | 20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (S) | |
| Storage temperature | 4°C to 30°C | |
| MonoBeads Columns* | ||
| Bed volume | PC 1.6/5 | 0.1 ml |
| 5/50 GL | 1 ml | |
| 4.6/100 PE | 1.7 ml | |
| 10/100 GL | 8 ml | |
| HR 16/10 | 20 ml | |
| Max. pressure | PC 1.6/5 | 50 bar (725 psi, 5 MPa) |
| 5/50 GL, 10/100 GL, 4.6/100 PE | 40 bar (580 psi, 4 MPa) | |
| HR 16/10 | 30 bar (435 psi, 3 MPa) | |
| Recommended flow rate | PC 1.6/5 | 0.01-0.4 ml/min |
| 5/50 GL, 4.6/100 PE | 0.5-3.0 ml/min | |
| 10/100 GL | 2.0-6.0 ml/min | |
| HR 16/10 | < 10 ml/min | |
| * Columns are not suitable for use with ÄKTAprime™ plus system.
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Tricorn high-performance columns are prepacked with MonoBeads ion exchange media for high-resolution purification and high capacity.
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The polishing step on Mono Q 5/50 GL shows different phosphoforms of the purified kinase and very high purity.
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